Gels were scanned using a computer GS-800 calibrated densitometer (Bio-Rad) and spots were detected and measured using PDQuest software V 6.2.0 (Bio-Rad). In order to minimize the limitations of 2D gel analysis resulting from gel to gel variation, and lack of uniformity in staining, the samples were exposed to the same conditions simultaneously for the first and second dimensions. Normalization using PDQuest was performed using the total density in image method to semi-quantify spot intensities and to minimize staining variation between gels.
Pdquest software v 6
Manufactured by Bio-Rad
Sourced in United States
PDQuest software V 6.2.0 is a data analysis software designed for two-dimensional gel electrophoresis and Western blot analysis. The software provides tools for image capture, image analysis, and quantification of protein spots or bands.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pdquest software v 6
1
2D Gel Electrophoresis Quantification
2
Quantitative 2D Gel Electrophoresis Analysis
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Gels were scanned using a computer GS-800 calibrated densitometer (Bio-Rad, Hercules, CA, USA) and spots were detected and quantified using PDQuest software V 6.2.0 (Bio-Rad, Hercules, CA, USA). In order to overcome several of the known limitations of 2D gel analysis that occur as a result of gel to gel variation, and also variability in staining14 (link), all samples were run simultaneously for the first and second dimensions. Normalization with PDQuest was performed using the total density in image method to semi-quantify spot intensities and to minimize staining variation between gels14 (link).
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