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Anti sox2 d6d9 rabbit monoclonal

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The Anti-SOX2 (D6D9 rabbit monoclonal) is a primary antibody specific for the SOX2 protein. SOX2 is a transcription factor that plays a critical role in the maintenance of pluripotency and self-renewal in embryonic stem cells. This antibody can be used to detect the expression of SOX2 in various cell and tissue samples using techniques such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions) S1699, by Agilent Technologies (2 mentions) Dab chromogen, by Agilent Technologies (2 mentions) Envision system, by Agilent Technologies (2 mentions) Nitrocellulose, by LI COR (2 mentions) Ripa buffer, by Bio-Rad (2 mentions) Sample buffer, by Bio-Rad (2 mentions) Donkey anti rabbit irdye 680, by LI COR (2 mentions) Odyssey infrared scanner, by LI COR (2 mentions) Anti beta actin clone ac 15, by Merck Group (2 mentions) Irdye 800cw goat anti mouse, by LI COR (2 mentions) β mercaptoethanol, by Merck Group (2 mentions)

4 protocols using anti sox2 d6d9 rabbit monoclonal

1

Immunohistochemical Staining of SOX2

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Hematoxylin and eosin staining was performed as previously described using a Sakura Tissue-Tek Prima Autostainer (Torrance, CA) [2 (link)]. For IHC staining, formalin-fixed, paraffin-embedded slides were deparaffinized in xylene and hydrated using graded ethanol washes. Tissues were treated with antigen retrieval buffer (S1699 from DAKO; Glostrup, Denmark) in a steamer for 20 min. Anti-SOX2 (D6D9 rabbit monoclonal, Cell Signaling Technology; Danvers, MA) was applied for 1 h at room temperature in a humidity chamber. Following TBS wash, antigen–antibody binding was detected with Envision+system (K4001, DAKO; Carpinteria, CA) and DAB+Chromogen (K3468, DAKO). Tissue sections were briefly immersed in hematoxylin for counterstaining and were cover-slipped. Staining quantitation was conducted by a genitourinary pathologist. Controls for specificity of anti-SOX2 staining using the D6D9 antibody are shown in Supplemental Figure 1.
de Wet L., Williams A., Gillard M., Kregel S., Lamperis S., Gutgesell L.C., Vellky J.E., Brown R., Conger K., Paner G.P., Wang H., Platz E.E., De Marzo A.M., Mu P., Coloff J.L., Szmulewitz R.Z, & Vander Griend D.J. (2022). SOX2 Mediates Metabolic Reprogramming of Prostate Cancer Cells. Oncogene, 41(8), 1190-1202.
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2

Western Blot Analysis of SOX2 Expression

Cited 1 time
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Whole-cell lysates of ≥100,000 cells were used per lane. Western blotting was performed as previously reported [2 (link)]. Briefly, cells were rinsed with cold PBS and scraped into RIPA buffer supplemented with protease inhibitors, sonicated twice, and re-suspended in 4X sample buffer (BioRad; Hercules, CA) supplemented with 10% beta-mercaptoethanol (Sigma-Aldrich; St. Louis MO). The Pierce BCA protein assay kit (Thermo Fisher Scientific; Grand Island, NY) was used to determine protein concentration. Protein (60 μg) was electrophoresed on 10% SDS-polyacrylamide gels and transferred to nitrocellulose (Odyssey, LI-COR Biosciences; Lincoln, NE) overnight at 4°C. Membranes were blocked overnight in 5% non-fat milk in TBS at 4°C. Primary antibodies used were: anti-SOX2 (D6D9 rabbit monoclonal, Cell Signaling Technologies) and anti-beta actin (clone AC-15, Sigma-Aldrich). Secondary antibodies were goat anti-mouse IRDye 800 CW or donkey anti-rabbit IRDye 680 (LI-COR Biosciences), and images were captured using an infrared Odyssey scanner (LI-COR Biosciences).
de Wet L., Williams A., Gillard M., Kregel S., Lamperis S., Gutgesell L.C., Vellky J.E., Brown R., Conger K., Paner G.P., Wang H., Platz E.E., De Marzo A.M., Mu P., Coloff J.L., Szmulewitz R.Z, & Vander Griend D.J. (2022). SOX2 Mediates Metabolic Reprogramming of Prostate Cancer Cells. Oncogene, 41(8), 1190-1202.
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3

Immunohistochemical Staining of SOX2

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hematoxylin and eosin staining was performed as previously described using a Sakura Tissue-Tek Prima Autostainer (Torrance, CA) [2 (link)]. For IHC staining, formalin-fixed, paraffin-embedded slides were deparaffinized in xylene and hydrated using graded ethanol washes. Tissues were treated with antigen retrieval buffer (S1699 from DAKO; Glostrup, Denmark) in a steamer for 20 min. Anti-SOX2 (D6D9 rabbit monoclonal, Cell Signaling Technology; Danvers, MA) was applied for 1 h at room temperature in a humidity chamber. Following TBS wash, antigen–antibody binding was detected with Envision+system (K4001, DAKO; Carpinteria, CA) and DAB+Chromogen (K3468, DAKO). Tissue sections were briefly immersed in hematoxylin for counterstaining and were cover-slipped. Staining quantitation was conducted by a genitourinary pathologist. Controls for specificity of anti-SOX2 staining using the D6D9 antibody are shown in Supplemental Figure 1.
de Wet L., Williams A., Gillard M., Kregel S., Lamperis S., Gutgesell L.C., Vellky J.E., Brown R., Conger K., Paner G.P., Wang H., Platz E.E., De Marzo A.M., Mu P., Coloff J.L., Szmulewitz R.Z, & Vander Griend D.J. (2022). SOX2 Mediates Metabolic Reprogramming of Prostate Cancer Cells. Oncogene, 41(8), 1190-1202.
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4

Western Blot Analysis of SOX2 Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates of ≥100,000 cells were used per lane. Western blotting was performed as previously reported [2 (link)]. Briefly, cells were rinsed with cold PBS and scraped into RIPA buffer supplemented with protease inhibitors, sonicated twice, and re-suspended in 4X sample buffer (BioRad; Hercules, CA) supplemented with 10% beta-mercaptoethanol (Sigma-Aldrich; St. Louis MO). The Pierce BCA protein assay kit (Thermo Fisher Scientific; Grand Island, NY) was used to determine protein concentration. Protein (60 μg) was electrophoresed on 10% SDS-polyacrylamide gels and transferred to nitrocellulose (Odyssey, LI-COR Biosciences; Lincoln, NE) overnight at 4°C. Membranes were blocked overnight in 5% non-fat milk in TBS at 4°C. Primary antibodies used were: anti-SOX2 (D6D9 rabbit monoclonal, Cell Signaling Technologies) and anti-beta actin (clone AC-15, Sigma-Aldrich). Secondary antibodies were goat anti-mouse IRDye 800 CW or donkey anti-rabbit IRDye 680 (LI-COR Biosciences), and images were captured using an infrared Odyssey scanner (LI-COR Biosciences).
de Wet L., Williams A., Gillard M., Kregel S., Lamperis S., Gutgesell L.C., Vellky J.E., Brown R., Conger K., Paner G.P., Wang H., Platz E.E., De Marzo A.M., Mu P., Coloff J.L., Szmulewitz R.Z, & Vander Griend D.J. (2022). SOX2 Mediates Metabolic Reprogramming of Prostate Cancer Cells. Oncogene, 41(8), 1190-1202.
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