Silica gel 60–120, Silica gel GF254, catechin, tricholoroacetic acid (TCA), donezepil, galantamine, and Tris-HCl were procured from Merck, Mumbai, India. Vincristine sulfate, dimethyl sulphoxide (DMSO), potassium ferricyanide, ferric chloride, and Folin-Ciocalteau reagent were from BDH chemicals Ltd., Poole, England. Acetylthiocholine and S-butyrylthiocholine and DTNB were purchased from Sigma-Aldrich, Darmstadt, Germany. Nutrient agar media was from Becton, Dickinson, and Company (Sparks, MD, USA). Petroleum ether and methanol were purchased from Duksan Pure Chemicals Company Limited (Ansan-si, Republic of Korea). The Institute of Nutrition and Food Science, University of Dhaka donated the cultures of bacteria and fungi. The other chemicals were of analytical grade.
Silica gel 60 120
Manufactured by Merck Group
Sourced in India, Germany
Silica gel 60–120 is a type of porous, high-surface-area silica material. It is used as a stationary phase in column chromatography and thin-layer chromatography applications.
Automatically generated - may contain errors
Lab products found in correlation
Tris hcl, by Merck Group (2 mentions)
Acetylthiocholine, by Merck Group (2 mentions)
S butyrylthiocholine, by Merck Group (2 mentions)
Silica gel gf254, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Ferric chloride, by Avantor (1 mentions)
Potassium ferricyanide, by Avantor (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Trichloroacetic acid tca, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Potassium ferricyanide, by Merck Group (1 mentions)
Aluminum chloride, by Merck Group (1 mentions)
Ammonium molybdate, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Donepezil, by Merck Group (1 mentions)
Galantamine, by Merck Group (1 mentions)
2 deoxy d ribose, by Merck Group (1 mentions)
3 protocols using silica gel 60 120
1
Acetylcholinesterase Inhibition Assay
2
Spectroscopic Characterization of Synthesized Compounds
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All
chemicals were obtained from Sigma-Aldrich Company and used as received. 1H and 13C NMR spectra were recorded on Bruker-Avance
III HD 500 and 125 MHz NMR instruments, respectively. Chemical shifts
are reported in parts per million (ppm) downfield from tetramethylsilane
(TMS) and are referenced to the residual proton/carbon in the NMR
solvent (CDCl3, 7.26:77.1 ppm; MeOD, 3.31:49.00 ppm; DMSO-d6, 2.50:39.5 ppm). All chromatographic purifications
were performed on silica gel (#60-120 or #100-200) obtained from Merck.
Thin-layer chromatography (TLC) was performed on precoated silica
gel 60 GF254 aluminum sheets (Merck) and visualized under
UV light (254 nm) by spraying an anisaldehyde–sulfuric acid
reagent followed by heating.
chemicals were obtained from Sigma-Aldrich Company and used as received. 1H and 13C NMR spectra were recorded on Bruker-Avance
III HD 500 and 125 MHz NMR instruments, respectively. Chemical shifts
are reported in parts per million (ppm) downfield from tetramethylsilane
(TMS) and are referenced to the residual proton/carbon in the NMR
solvent (CDCl3, 7.26:77.1 ppm; MeOD, 3.31:49.00 ppm; DMSO-d6, 2.50:39.5 ppm). All chromatographic purifications
were performed on silica gel (#60-120 or #100-200) obtained from Merck.
Thin-layer chromatography (TLC) was performed on precoated silica
gel 60 GF254 aluminum sheets (Merck) and visualized under
UV light (254 nm) by spraying an anisaldehyde–sulfuric acid
reagent followed by heating.
3
Screening of Phytochemical and Bioactive Potential
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2,2′-Diphenyl-1-picrylhydrazyl (DPPH), 5,5′-dithio-bis-(2-nitro) benzoic acid (DTNB), thiobarbituric acid (TBA), 2-deoxy-D-ribose, acetylthiocholine, S-butyrylthiocholine, donepezil, and galantamine were procured from Sigma-Aldrich, Germany. silica gel GF264, silica gel 60-120, Folin-Ciocalteu reagent, Tris-HCl, aluminum chloride, potassium ferricyanide, ammonium molybdate, trichloroacetic acid (TCA), and triton X-100 were from Merck, Darmstadt, Germany. Catechin, gallic acid, and ascorbic acid were purchased from Wako Pure Chemical Company Ltd., Osaka, Japan. Petroleum ether, chloroform, ethylacetate, and methanol were obtained from Active Fine Chemicals Limited, Dhaka, Bangladesh. Unless specified, all other chemicals were of analytical grade.
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