Histone proteins were purchased from the following commercial sources: unfractionated whole histone from calf thymus (Sigma-Aldrich; H9250) and histone H3 from calf thymus (Roche; 11034758001). Proteases qualified for use in mass spectrometry sample preparation are as follows: trypsin (Promega; V5111), chymotrypsin (Promega; V1061), and clostripain (Promega; V1881). Standards used in LC-MS/MS are as follows: L -lysine monohydrochloride (Sigma-Aldrich; L-5626), Nε-monomethyl-L -lysine hydrochloride (Sigma-Aldrich; 04,685), Nε,Nε -dimethyl-L -lysine monohydrochloride (Sigma-Aldrich; 19,773), Nε,Nε,Nε-trimethyl-lysine hydrochloride (Sigma-Aldrich; T1660), L -arginine hydrochloride (Arg, Sigma-Aldrich; A-5131), NG-monomethyl-L -arginine monoacetate salt (Sigma-Aldrich; 475,886), NG,NG-dimethylarginine dihydrochloride (Sigma-Aldrich; D4268), NG,N′G-dimethyl-L -arginine dihydrochloride (Santa Cruz; sc-222070), L -histidine hydrochloride monohydrate (His, Wako; 084–00702), 3-methyl-L -histidine (Sigma-Aldrich; M9005), 1-methyl-L-histidine (Sigma-Aldrich; 67,520), Nω-propyl-L -arginine hydrochloride (Wako; 512–27111).
1 methyl l histidine
Manufactured by Merck Group
1-methyl-L-histidine is a chemical compound that is used as a laboratory reagent. It is a derivative of the amino acid histidine, with a methyl group attached to the imidazole ring. This compound is utilized in various biochemical and analytical applications, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Chymotrypsin, by Promega (1 mentions)
L lysine monohydrochloride, by Merck Group (1 mentions)
3 methyl l histidine, by Merck Group (1 mentions)
Histone h3, by Roche (1 mentions)
Trypsin, by Promega (1 mentions)
Glycochenodeoxycholic acid, by Merck Group (1 mentions)
Naringenin, by Extrasynthese (1 mentions)
Procyanidin b2, by Merck Group (1 mentions)
L tryptophan, by Merck Group (1 mentions)
L phenylalanine, by Merck Group (1 mentions)
Gradient a10 system, by Merck Group (1 mentions)
Epicatechin, by Merck Group (1 mentions)
2 protocols using 1 methyl l histidine
1
Histone Modification Standards for Mass Spectrometry
2
Commercially Obtained Chemicals Analysis
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The following chemicals were obtained commercially: L-phenylalanine, 1-methyl-L-histidine, L-tryptophan, 20-deoxyguanosine, glycochenodeoxycholic acid, (-)-epicatechin and procyanidin B2 (Sigma-Aldrich, St Louis, MO), 4-hydroxyhippuric acid (PhytoLab GmbH & Co., KG) and naringenin (Extrasynthèse, Genay, France). HPLC-grade acetonitrile and formic acid were purchased from Scharlau Chemie S.A. (Barcelona, Spain). Ultrapure water (Milli-Q) was obtained from a Milli-Q Gradient A10 system (Millipore, Bedford, MA).
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