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Geneticin g418 sulfate

Manufactured by Corning
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Geneticin (G418) Sulfate is a broad-spectrum antibiotic used as a selectable marker in cell and molecular biology. It inhibits protein synthesis, allowing for the selection of cells that have been successfully transformed with a gene of interest.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions) Glutamine, by Merck Group (2 mentions) K gluconate, by Merck Group (2 mentions) Fastdigest hindiii, by Thermo Fisher Scientific (2 mentions) Phenol red free neurobasal medium, by Thermo Fisher Scientific (2 mentions) Fastdigest nhei, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle medium high glucose, by Merck Group (2 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (2 mentions) Mgcl2, by Merck Group (2 mentions) Poly d lysine hydrobromide, by Merck Group (2 mentions) Hepes, by Merck Group (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Penicillin streptomycin, by Merck Group (2 mentions) Poly d lysine, by Merck Group (2 mentions) Sucrose, by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Cacl2, by Merck Group (2 mentions)

Spelling variants of this product

G418 sulfate (42 citations) Geneticin (11 citations) Geneticin (G418) (2 citations)

2 protocols using geneticin g418 sulfate

1

Primary Neuron Culture and Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Basic chemical reagents include: NaCl (S3014, Sigma-Aldrich), sucrose (S0389, Sigma-Aldrich), glucose (G8270, Sigma-Aldrich), HEPES (H3375, Sigma-Aldrich), KCl (P9541, Sigma-Aldrich), MgSO4 (M2643, Sigma-Aldrich), K-gluconate (P1847, Sigma-Aldrich), EGTA (E3889, Sigma-Aldrich), MgCl2 (M9272, Sigma-Aldrich), CaCl2 (223506, Sigma-Aldrich), KOH (P250, Thermo Fisher) and NaOH (S5881, Sigma-Aldrich).
Cell culture reagents include: high-glucose Dulbecco’s Modified Eagle Medium (D1145, Sigma-Aldrich), fetal bovine serum (F2442, Sigma-Aldrich), glutamine (G7513, Sigma-Aldrich), Penicillin/Streptomycin (P4333, Sigma-Aldrich), Geneticin (G418) Sulfate (30–234-CR, Corning), 30–70 kD poly-D-lysine (P7886, Sigma-Aldrich), 300 kD poly-D-lysine hydrobromide (P7405, Sigma-Aldrich) and phosphate-buffered saline (PBS, SH302560, HyClone, GE Healthcare).
Primary neuronal culture reagents include: phenol-red-free Neurobasal medium (12348017, Gibco), B-27 (17504044, Gibco), Glutamax (35050061, Gibco) and cytosine β-D-arabinofuranoside (C1768, Sigma-Aldrich).
Transfection and cloning reagents include: jetPRIME (114–15, Polyplus Transfection), FuGENE HD transfection reagent (E2311, Promega), lipofectamine 2000 (11668019, Thermo Fisher Scientific), FastDigest NheI (FD0974, Thermo Fisher Scientific) and FastDigest HindIII (FD0504, Thermo Fisher Scientific).
Liu Z., Lu X., Villette V., Gou Y., Colbert K.L., Lai S., Guan S., Land M.A., Lee J., Assefa T., Zollinger D.R., Korympidou M.M., Vlasits A.L., Pang M.M., Su S., Cai C., Froudarakis E., Zhou N., Patel S.S., Smith C.L., Ayon A., Bizouard P., Bradley J., Franke K., Clandinin T.R., Giovannucci A., Tolias A.S., Reimer J., Dieudonné S, & St-Pierre F. (2022). Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell, 185(18), 3408-3425.e29.
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2

Primary Neuron Culture and Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Basic chemical reagents include: NaCl (S3014, Sigma-Aldrich), sucrose (S0389, Sigma-Aldrich), glucose (G8270, Sigma-Aldrich), HEPES (H3375, Sigma-Aldrich), KCl (P9541, Sigma-Aldrich), MgSO4 (M2643, Sigma-Aldrich), K-gluconate (P1847, Sigma-Aldrich), EGTA (E3889, Sigma-Aldrich), MgCl2 (M9272, Sigma-Aldrich), CaCl2 (223506, Sigma-Aldrich), KOH (P250, Thermo Fisher) and NaOH (S5881, Sigma-Aldrich).
Cell culture reagents include: high-glucose Dulbecco’s Modified Eagle Medium (D1145, Sigma-Aldrich), fetal bovine serum (F2442, Sigma-Aldrich), glutamine (G7513, Sigma-Aldrich), Penicillin/Streptomycin (P4333, Sigma-Aldrich), Geneticin (G418) Sulfate (30–234-CR, Corning), 30–70 kD poly-D-lysine (P7886, Sigma-Aldrich), 300 kD poly-D-lysine hydrobromide (P7405, Sigma-Aldrich) and phosphate-buffered saline (PBS, SH302560, HyClone, GE Healthcare).
Primary neuronal culture reagents include: phenol-red-free Neurobasal medium (12348017, Gibco), B-27 (17504044, Gibco), Glutamax (35050061, Gibco) and cytosine β-D-arabinofuranoside (C1768, Sigma-Aldrich).
Transfection and cloning reagents include: jetPRIME (114–15, Polyplus Transfection), FuGENE HD transfection reagent (E2311, Promega), lipofectamine 2000 (11668019, Thermo Fisher Scientific), FastDigest NheI (FD0974, Thermo Fisher Scientific) and FastDigest HindIII (FD0504, Thermo Fisher Scientific).
Liu Z., Lu X., Villette V., Gou Y., Colbert K.L., Lai S., Guan S., Land M.A., Lee J., Assefa T., Zollinger D.R., Korympidou M.M., Vlasits A.L., Pang M.M., Su S., Cai C., Froudarakis E., Zhou N., Patel S.S., Smith C.L., Ayon A., Bizouard P., Bradley J., Franke K., Clandinin T.R., Giovannucci A., Tolias A.S., Reimer J., Dieudonné S, & St-Pierre F. (2022). Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell, 185(18), 3408-3425.e29.
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