LC – MS grade acetonitrile, high-performance liquid chromatography (HPLC) grade methanol, and ACS grade methanol were obtained from Fisher Scientific (Fair Lawn, NJ). Food grade ethanol was purchased from Decon Labs (King of Prussia, PA). SP-70 resin was obtained from Fisher Scientific (Fair Lawn, NJ). HPLC reverse phase column was purchased from Waters (Milford, MA). Flash column chromatography was performed using a Buchi Pure C-810 (Flawil, Switzerland) equipped with reverse phase column (EcoFlex C18, 220 g). Sephadex LH-20 gel was purchased from GE Healthcare (Piscataway, NJ). Quinine monohydrochloride dihydrate and caffeine were purchased from Sigma-Aldrich (St. Louis, MO). 1H and 13C NMR data were collected on a Bruker Avance II 600 MHz spectrometer at the University of Florida. A HPLC system from Jasco (Tokyo, Japan) was utilized at a flow rate of 3 mL/min with UV detection at 210 and 254 nm using an X-select C18 column (10 × 250 mm, 5 μm particle size; Waters, Milford, MA). The Lyovapor L-300 from Buchi (Flawil, Switzerland) was used as a high-vacuum system to remove the solvents.
Quinine monohydrochloride dihydrate
Manufactured by Merck Group
Sourced in United States
Quinine monohydrochloride dihydrate is a chemical compound used as a laboratory reagent. It is a crystalline solid that is soluble in water and organic solvents. Quinine monohydrochloride dihydrate is commonly used in various analytical and research applications, but its specific core function is not provided in order to maintain an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Caffeine, by Merck Group (2 mentions)
Acs grade methanol, by Thermo Fisher Scientific (1 mentions)
Lyovapor l 300, by Büchi (1 mentions)
Avance 2 600, by Bruker (1 mentions)
Xselect c18 column, by Waters Corporation (1 mentions)
Sephadex lh 20 gel, by GE Healthcare (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Lc ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Hplc system, by Jasco (1 mentions)
Pancreatin from porcine pancreas, by Merck Group (1 mentions)
Pepsin from porcine gastric mucosa, by Merck Group (1 mentions)
Dppiv glo protease assay, by Promega (1 mentions)
α amylase from porcine pancreas, by Merck Group (1 mentions)
Tannic acid, by Merck Group (1 mentions)
Sucrose octaacetate, by Merck Group (1 mentions)
Magnesium sulfate heptahydrate, by Merck Group (1 mentions)
Sodium bicarbonate, by Thermo Fisher Scientific (1 mentions)
L tryptophan, by Merck Group (1 mentions)
L phenylalanine, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
4 protocols using quinine monohydrochloride dihydrate
1
Purification and Characterization of Bioactive Compounds
2
Phaseolus vulgaris Digestibility and DPP-IV Inhibition
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Raw black bean (Phaseolus vulgaris L.) San Luis cultivar (2017) from Sombrerete, Zacatecas, Mexico (23°37′53.9″ N 103°38′29.5″ W). The dry beans were stored at 4 °C until use. Pepsin from porcine gastric mucosa (EC 232-629-3), pancreatin from porcine pancreas (EC 232-468-9), α-Amylase from porcine pancreas (EC 232-565-6), quinine monohydrochloride dihydrate (90%), and tannic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). DPP-IV Glo® protease assay was purchased from Promega (Madison, WI, USA). Gum arabic (GA; Acacia senegal) was purchased from Reasol (Mexico City, Mexico) (2.3% protein, 3.7% ash content, 0.1% fat, and 93.8% carbohydrates content). Whey protein concentrate (WPC) was purchased from Hilmar (Vilher, Jalisco, Mexico) (84.1% protein, 5.1% ash content, 0.7% fat, and 10.0% carbohydrates content). Canola oil was purchased from a local market (Jalisco, Mexico). Culture YO-PROX 753 was purchased from Bioprox (Mexico City, Mexico). All other reagents were analytical grade.
3
Purification and Characterization of Cyclic Dipeptides
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Caffeine, (+)-catechin, (-)-epicatechin, naringin, sucrose octaacetate, L-tryptophan, L-phenylalanine, magnesium sulfate heptahydrate, sodium bicarbonate, quinine monohydrochloride dihydrate and methanol were purchased in food grade or primary reference standard quality from Sigma-Aldrich (St. Louis, MO). Urea was purchased from Spectrum Chemical (Gardena, CA). sodium bicarbonate was purchased from Fisher Chemical (Fair Lawn, NJ). Cyclo(-leu-pro), cyclo(-pro-val), cyclo(-phe-pro) were synthetized by Bachem Americas (Torrance, CA) in high purity (>99%) and further underwent Solid Phase Extraction (SPE) to ensure high purity and removal of any residual solvent. Briefly, 0.6 g of material were dissolved in 200 mL of 90/10 water/methanol and loaded onto a 6g/35cc HLB Prime cartridge (Waters, Milford, MA). The cartridge was further washed with 60 mL of 95/5 water/methanol and elution was carried out with 60 mL of 95/5 water/methanol. Sample was freed from solvent and freeze-dried twice to ensure safety for consumption.
4
Analytical Reagents and E-Tongue Arrays
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In all assays, deionized type II water was used. Folin-Ciocalteau, sodium carbonate, n-hexane, methanol and ethanol were of analytical grade (Panreac, Barcelona). Quinine monohydrochloride dihydrate was purchased to Sigma-Aldrich (p.a., minimum pur-ityZ90%). For E-tongue arrays construction, all reagents were from Fluka (minimum purity Z97%): plasticizers (bis(1-butylpentyl) adipate, dibutyl sebacate, 2-nitrophenyl-octylether, tris(2-ethylhexyl)phosphate and dioctyl phenylphosphonate) and additives (octadecylamine, oleyl alcohol, methyltrioctylammonium chloride and oleic acid). High molecular weight polyvinyl chloride was used as the supporting polymer (Fluka).
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