Cell lines were purchased from the cell culture center at Chinese Academy of Medical Sciences and Peking Union Medical College. All cells were grown at 37 ℃ in a humidified atmosphere containing 5% CO2. HBE and H1299 cells were grown in RPMI-1640 medium (Sigma-Aldrich, St. Louis, USA, #R8758) supplemented with 10% fetal bovine serum (FBS) (Sigma, St. Louis, USA, #F3885). A549 cells were grown in the F12K Kaighn’s modification medium (Gibco, Thermo Fisher Scientific, Waltham, USA, #21127022) supplemented with 10% FBS. Panc-1 cells were grown in DMEM medium (Sigma-Aldrich, #D5796) supplemented with 10% FBS. MD-MBA-231 cells were grown in L-15 medium (HyClone, GE Healthcare Life Sciences, Pittsburgh, USA, #SH30525.01) supplemented with 10% FBS.
F12k kaighn s modification medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
F12K Kaighn's modification medium is a cell culture medium used for the growth and maintenance of various cell types. It is a modification of the Ham's F12 medium and is designed to support the nutritional requirements of specific cell lines. The core function of this medium is to provide a balanced and optimized environment for cell proliferation and viability.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dmem medium, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Penicillin streptomycin, by Euroclone (1 mentions)
Cabozantinib, by Cayman Chemical (1 mentions)
Trypsin 0.05, by Merck Group (1 mentions)
Vandetanib, by Cayman Chemical (1 mentions)
2 protocols using f12k kaighn s modification medium
1
Cell Culture Conditions for Cancer Cell Lines
2
Evaluation of Vandetanib and Cabozantinib in MTC Cell Lines
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Vandetanib (VAN), and cabozantinib (CAB) were provided by Cayman Chemicals (Ann Arbor, MI, USA). Stock solutions (4 mM) were made in 100% dimethyl sulfoxide (DMSO) and diluted with culture media before use. Two human medullary thyroid carcinoma (MTC) cell lines, TT and MZ-CRC-1, were kindly provided by Prof. Lips (Utrecht, the Netherland). Cells were maintained at 37 °C in 5% CO2 and cultured in T75 flasks filled with 10 mL of F-12K Kaighn’s modification medium (Gibco™ Thermo Fisher Scientific, Waltham, MA, USA). Media was supplemented with 10% heat-activated fetal bovine serum (FBS) (Invitrogen™ Thermo Fisher Scientific, Waltham, MA, USA) and 105 U·L−1 penicillin/streptomycin (EuroClone™, Milan, Italy). Cells were harvested by trypsinization (Trypsin 0.05% and EDTA 0.02%) (Sigma-Aldrich® Merck KGaA, Darmstadt, Germany), resuspended in complete medium, then counted through an optical microscope using a standard haemocytometer before plating. Cells used in all experiments were below 5 passages. All in vitro experiments were monitored for up to 6 days of drug incubation. We performed long-term treatments due to the slow doubling time (about 4 days) of the MTC cell lines.
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