The human GBC cell lines GBC-SD and SGC-996 were purchased from the Shanghai Institute for Biological Science, Chinese Academy of Science (Shanghai, China), and the NOZ, EH-GB1, and OCUG-1 cell lines were obtained from the Health Science Research Resources Bank (Osaka, Japan). The GBC-SD, NOZ, EH-GB1, and OCUG-1 cell lines were cultured in high-glucose DMEM (Gibco, NY, USA), and the SGC-996 cell line was cultured in Rosewell Park Memorial Institute (RPMI) 1640 medium (HyClone, Logan, UT, USA). All of the above media were supplemented with 10% foetal bovine serum (Gibco, NY, USA), and all of the above cell lines were cultured at 37 °C in a humidified atmosphere with 5% CO2.
Eh gb1
Manufactured by Health Science Research Resources Bank
Sourced in Japan, China
The EH-GB1 is a laboratory equipment designed for general purpose use. It serves as a basic tool for various research applications. The core function of the EH-GB1 is to provide a stable and controllable environment for conducting experiments and analyses. No further details on the intended use or specific applications are provided.
Automatically generated - may contain errors
Lab products found in correlation
Eh gb1, by Thermo Fisher Scientific (3 mentions)
High glucose dmem, by Thermo Fisher Scientific (2 mentions)
Gbc sd, by Thermo Fisher Scientific (2 mentions)
Ocug 1, by Thermo Fisher Scientific (2 mentions)
Ocug 1, by Health Science Research Resources Bank (2 mentions)
Gbc sd, by National Collection of Authenticated Cell Cultures (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
William s medium, by Thermo Fisher Scientific (1 mentions)
Ly294002 s1105, by Selleck Chemicals (1 mentions)
Ly294002, by Selleck Chemicals (1 mentions)
Ehgb 2, by Thermo Fisher Scientific (1 mentions)
William s e medium, by Lonza (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
5 protocols using eh gb1
1
Cultivation of Human Gallbladder Cancer Cell Lines
2
Culturing Human Gallbladder Cell Lines
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The human GBC-SD cell lines and the normal biliary epithelia cell line HIBEC were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and EH-GB-1 and NOZ cell lines were purchased from the Health Science Research Resources Bank (Osaka, Japan). The another human GBC cell line, SGC-996, was obtained from the Tongji University School of Medicine (Shanghai, China). The GBC-SD and EH-GB-1 cells were cultured in high-glucose DMEM (Gibco, Grand Island, NY, USA), NOZ cell line was maintained in William's medium (Gibco, Grand Island, NY, USA), and SGC-996 cell line was grown in RPMI 1640 (Hyclone, Logan, TX, USA) in a humidified incubator (5% CO2, 37 oC). Cells were cultured in recommended medium supplemented with 10% (v/v) fetal bovine serum (Gibco, USA), 100 μg/mL streptomycin and 100 U/mL penicillin (Hyclone).
LY294002 (S1105) was purchased from Selleck Chemicals (Houston, TX, USA), and GBC-SD and EH-GB-1 cell lines were treated with LY294002 at 20 μM for 12 h.
LY294002 (S1105) was purchased from Selleck Chemicals (Houston, TX, USA), and GBC-SD and EH-GB-1 cell lines were treated with LY294002 at 20 μM for 12 h.
3
Cell Culture Conditions for Gallbladder Carcinoma
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The GBC lines used in this study were as follows: GBC-SD, SGC-996 were purchased from Shanghai Institute for Biological Science, Chinese Academy of Science (Shanghai, China). NOZ, OCUG-1, EHGB-1, EHGB-2 were purchased from the Health Science Research Resources Bank (Osaka, Japan). GBC-SD, EHGB-1, EHGB-2, OCUG-1 cells were cultured separately in DMEM (Gibco) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin–streptomycin (Gibco). NOZ cells were cultured in William’s medium E (Lonza) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin–streptomycin (Gibco). Contrastingly, SGC-996 cells were cultured in RPMI 1640 medium (Hyclone) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin–streptomycin (Gibco). All of above cells were cultured in their respective media in a humidified incubator at 37 °C with 5% CO2.
4
Establishment and characterization of gallbladder cancer cell lines
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Human tumor cell lines GBC-SD were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), SGC-996 was obtained from Tongji University (Shanghai, China). The other human GBC cell lines EH-GB1 and NOZ were purchased from the Health Science Research Resources Bank (Osaka, Japan). GBC-SD, EH-GB1, and SGC-996 were cultured in DMEM, and NOZ cell line was cultured in William’s medium. All cells were cultured in their corresponding media supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified atmosphere of 95% air and 5% CO2, all the cell line without mycoplasma contamination. All 49 GBC tissues were collected from patients who underwent radical surgery at the Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. We retrospectively obtained clinicopathological data from these patients, including age, sex, T stage regional lymph node status, TNM stage, and differentiation. The usage of these specimens and the patient data were approved by the Ethics Committee of the Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine.
5
Establishment and Characterization of Gallbladder Carcinoma Cell Lines
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Human tumor cell lines GBC-SD were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), SGC-996 was obtained from Tongji university (Shanghai, China). The other human GBC cell lines EH-GB1 and NOZ were purchased from the Health Science Research Resources Bank(Osaka, Japan). GBC-SD, EH-GB1, and SGC-996 were cultured in DMEM and NOZ cell line was cultured in William's medium. All cells were cultured in their corresponding media supplemented with 10% fetal bovine serum (FBS)at 37°C in a humidified atmosphere of 95% air and 5% CO 2 .All 49 GBC tissues were collected from patients who underwent radical surgery at the Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. We retrospectively obtained clinicopathological data from these patients, including age, sex, T stage regional lymph node status, TNM stage, and differentiation. The usage of these specimens and the patient data were approved by the Ethics Committee of the Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine.
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