Alp elisa kit

Manufactured by Nanjing Jiancheng

Sourced in China

The ALP ELISA kit is a laboratory equipment product designed for the quantitative determination of alkaline phosphatase (ALP) levels in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure ALP concentrations accurately.

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Lab products found in correlation

Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ALP kit ELISAs

2 protocols using alp elisa kit

Alkaline Phosphatase Activity in C3H10T1/2 Cells

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C3H10T1/2 cells were seeded in 96-well culture plates (1 × 10⁴ cells/plate) and cultured in 0.2 ml of a DMEM/F12k medium (HyClone, USA) supplemented with 10 % fetal calf serum (FCS, Gibco, USA) and 4 μl of protein extracts that were obtained after 2, 4, 6, and 8 weeks. Medium without fetal bovine serum was used as the control. Alkaline phosphatase (ALP) activity was determined after 1, 3, 6, and 9 days using an ALP ELISA kit (Jiancheng, Nanjing, China). Enzyme activity is expressed as absorbance per microgram of protein per minute (μg/min/μgprot).

Wang X., Wei F., Luo F., Huang K, & Xie Z. (2015). Induction of granulation tissue for the secretion of growth factors and the promotion of bone defect repair. Journal of Orthopaedic Surgery and Research, 10, 147.

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Alkaline Phosphatase Activity Assay

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Cells cultured for 14 days were assessed for ALP activity. ALP staining was performed using the nitroblue tetrazolium (NBT) and 5-bromo-4chloro-3-indolyl phosphate (BCIP) (NBT/BCIP) staining kit (C3206; Beyotime) according to the manufacturer's instructions. And ALP quantification was performed using the ALP ELISA kit (A059-2; Nanjing Jiancheng Bioengineering Institute).

Guo Y., Ma S., Wang D., Mei F., Guo Y., Heng B.C., Zhang S., Huang Y., Wei Y., He Y., Liu W., Xu M., Zhang X., Chen L, & Deng X. (2024). HtrA3 paves the way for MSC migration and promotes osteogenesis. Bioactive Materials, 38, 399-410.

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