C57bl 6j

Animal experiments were approved by the Institutional Animal Care and Use Committee of Kyungpook National University. C57BL/6J (male, 8- to 9-week-old) mice were obtained from Central Lab Animal Inc. Mice were injected intraperitoneally with STZ (50 mg/kg; Sigma-Aldrich, dissolved in 0.1 M citrate buffer, pH 4.5). Fasting blood glucose levels were monitored on day 6 after STZ injection, and mice with fasting blood glucose levels of >300 mg/dl were included in this study. Excisional dorsal full-thickness skin wounds (6 mm in diameter) were induced in the center of each dorsal skin on day 7 after STZ injection, and mice were administered either vehicle or bempedoic acid (10 mg/kg; MedChemExpress, dissolved in corn oil) on every 2 d. The wound area ratio was quantified by calculating the percentage of wound area compared to initial wound area.

All animal experiments were performed according to the National Institutes of Health's Guide for the Care and Use of Laboratory Animals, with approval of the Animal Experiment Ethics Committee of the Catholic University of Korea, College of Medicine. 4‐week‐old C57BL/6J and ob/ob mice were purchased from Central Lab Animal Inc (Korea). 4‐week‐old C57BL/6 mice were purchased from Orient Bio (Korea). Mice were maintained in a specific pathogen‐free facility with 12 h light and 12 h dark cycles.

Five-week-old male db/db and db/m mice from the C57BL/6J background were purchased from Central Lab Animal, Inc. (Seoul, South Korea) and maintained in the animal facility at Gyeongsang National University. Experiments were performed in accordance with the National Institutes of Health Guidelines on the Use of Laboratory Animals (GNU-130306-M0021). All experimental protocols were approved by the ethics committee of Gyeongsang National University. Mice were individually housed with an alternating 12-h light/dark cycle. Starting at five weeks of age, mice were fed a normal standard diet chow (ND) for five weeks. Mice were then randomly divided into three groups, db/m, db/db and db/db+CR (n = 10 per group), at 10 weeks of age. For the db/db+CR group, db/db mice were transferred to individual cage and received a restricted amount of food (2 g/day) for 12 weeks as previously described44 (link). The db/m and db/db mice were given free access to food. Mice were weighed four times monthly and immediately prior to their sacrifice at 22 weeks of age.