Agilent 1290 infinity diode array detector

Liquid samples, including 50 mM propionic acid as internal standard, were analyzed with respect to glucose and organic acids using an Agilent 1290 LC II system (Agilent, USA), with an Agilent 1290 Infinity Binary Pump, Agilent 1290 Infinity Autosampler, Agilent 1290 Infinity diode array detector operated at 210 nm, and an Agilent 1260 Infinity RI detector operated at 45 °C [3 (link)]. The HPLC was operated with an Aminex HPX-97H (Bio-Rad, USA) column at 60 °C and 0.008 mM H2SO4 as mobile phase at 0.8 mL/min as flow rate.
Analysis of ethanol and ethyl acetate in the liquid phase was carried out by an Agilent 7890B gas chromatograph (Agilent, USA) equipped with a flame ionization detector (GC-FID) and an Agilent 7693 autosampler [9 (link)]. Samples were injected into a NukolTM column (30 m × 0.53 mm, 1.0 μm coating, Supelco, USA). Column temperature was maintained at 50 °C for 2 min, then increased to 200 °C at the rate of 50 °C/min, with a split ratio of 10. As internal standard 2 mM 1-butanol was added.
Online measurements of volatile compounds and gases removed from the reactor vessel by gas stripping were performed with an δB Process Mass Spectrometer (MS, Thermo Scientific™, USA) [3 (link)].

Glucose and organic acids were analysed by HPLC on an Agilent 1290 LC II system, equipped with an Agilent 1290 Infinity Binary Pump, Agilent 1290 Infinity Autosampler, Agilent 1290 Infinity diode array detector operated at 210 nm, and an Agilent 1260 Infinity RI detector operated at 45 °C. Either an Aminex HPX-87H (Bio-Rad) or a Rezex ROA-Organic Acid H + (Phenomenex) column was used with a mobile phase of 0.008 mM H₂SO₄. The HPLC was operated at 0.8 mL/min and 60 °C. Propionic acid (50 mM) was used as an internal standard.
Ethyl acetate and ethanol in liquid samples were measured by an Agilent 7890B gas chromatograph equipped with a flame ionisation detector (GC-FID) and an Agilent 7693 autosampler. Samples were analysed by injecting 0.5 μL of liquid sample onto a Nukol^TM column (30 m × 0.53 mm, 1.0 μm coating, Supelco). The column temperature was maintained at 50 °C for 2 min and increased to 200 °C at a rate of 50 °C/min. The split ratio was 10. 1-Butanol (2 mM) was used as an internal standard.