RNA was extracted with TRIzol Reagent (ThermoFisher) and reverse transcribed using Transcriptor First Strand cDNA Synthesis Kit for RT-PCR following the indications of the manufacturer (Thermo-Fisher). Real-time PCR was conducted with SYBR Green Master on a MyiQ Real-Time PCR System (Bio-Rad). Primer oligonucleotide sequences are available on request. Validation of amplification efficiency was performed for each pair of primers (29 (link)). PCR thermocycling parameters were 95°C for 10 min, 40 cycles of 95°C for 15 s, and 60°C for 1 min. Each sample was run in duplicate and was normalized vs. 36B4. The fold induction (FI) was determined in a ΔΔCt based fold-change calculation.
Transcriptor first strand cdna synthesis kit for rt pcr
Manufactured by Thermo Fisher Scientific
The Transcriptor First Strand cDNA Synthesis Kit is a reagent kit for the conversion of RNA into complementary DNA (cDNA) for use in reverse transcription polymerase chain reaction (RT-PCR) applications. The kit contains all the necessary components for the first-strand cDNA synthesis reaction, including reverse transcriptase enzyme, reaction buffer, and dNTPs.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using transcriptor first strand cdna synthesis kit for rt pcr
1
Real-Time qRT-PCR for Gene Expression
2
Quantitative Real-Time PCR Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA was extracted with TRIzol Reagent (Thermo-Fisher, Carlsbad, CA) and reverse transcribed using Transcriptor First Strand cDNA Synthesis Kit for RT-PCR following the indications of the manufacturer (Thermo-Fisher). Real-time PCR was conducted with SYBR Green Master (Thermo-Fisher) on a MyiQ Real-Time PCR System (Bio-Rad). Primer oligonucleotide sequences are available on request. Validation of amplification efficiency was performed for each pair of primers [30] (link). PCR thermocycling parameters were 95 °C for 10 min, 40 cycles of 95 °C for 15 s, and 60 °C for 1 min. Each sample was run in duplicate and was normalized vs. 36B4. The fold induction (FI) was determined in a ΔΔCt based fold-change calculation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!