RAP and PLGA (MW 90000, 50:50) were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, Chia). 1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) was purchased from Biotium Inc. (Fremont, US). DiO, DAPI, Cell Total Protein Extraction kits and Membrane Protein Extraction kits were supplied by Beyotime Institute of Biotechnology (Jiangsu, China). The CellTiter 96TM AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Thermo Fisher Scientific (SanJose, CA, USA). Lipopolysaccharide (LPS) was purchased from Solarbio (Beijing, China). The mouse glycoprotein ELISA kit, mouse TNF-α ELISA kit and mouse IL-6 ELISA kit were purchased from Wuhan Colorful Gene Biological Technology Co., Ltd. (Wuhan, China). LysoTracker Green was purchased from Yeasen Biotech Co. Ltd. (Shanghai China). Ultrapure water with a resistivity of 18.2 MΩ·cm was used throughout the experiments.
1 19 dioctadecyl 3 3 39 39 tetramethylindodicarbocyanine perchlorate did
Manufactured by Biotium
Sourced in United States
1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) is a lipophilic carbocyanine dye. It is a fluorescent probe that can be used to label cell membranes and lipid-rich structures.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Beyotime (2 mentions)
Cell total protein extraction kits, by Beyotime (2 mentions)
Celltiter 96tm aqueous one solution cell proliferation assay, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharide lps, by Solarbio (1 mentions)
Membrane protein extraction kit, by Beyotime (1 mentions)
Lysotracker green, by Yeasen (1 mentions)
Goat anti rabbit igg h l alexa fluor 488 ab150077, by Abcam (1 mentions)
N acetyl l cysteine, by Beyotime (1 mentions)
2 protocols using 1 19 dioctadecyl 3 3 39 39 tetramethylindodicarbocyanine perchlorate did
1
Nanoparticle Fabrication and Characterization
2
DiD-Based Fluorescence Imaging Assay
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1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) was purchased from Biotium Inc. (Fremont, USA). DAPI, cell total protein extraction kits, and N-acetyl-l -cysteine (NAC) were provided by Beyotime Institute of Biotechnology (Jiangsu, China). Antibodies against CD31 (ab28364) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) were obtained from Abcam. l -Ascorbic acid (VC) was purchased from Aladdin (Shanghai, China). Malondialdehyde (MDA), superoxide dismutase (SOD), and monocyte chemotactic protein 1 (MCP-1) enzyme-linked immunosorbent assay (ELISA) kits were provided by KeyGen Biotech (Nanjing, China).
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