Uv 2600 spectrophotometer

Synthetic procedures are described below. All other chemicals were purchased from commercial suppliers and used without further purication. Dry solvents were puried using an MBraun SPS-800. NMR spectra were recorded on a Bruker DRX 500, AMX 400 or DRX 300, ESI-MS data were obtained from an HR-ToF Bruker Daltonik GmbH Impact II. UV-vis spectra were recorded on a Shimadzu UV-2600 spectrophotometer and uorescence measurements were performed on an Edinburgh Instruments Spectrouorometer FS5. Synthesis PDI-Py 2 ligand 50 and supramolecular heteroleptic square 2, 44 were prepared according to literature procedures.
Host-guest binding studies 1 H-NMR titrations. To a stock solution of 2 (600 mL, 0.42 mM) or 1 (600 mL, 0.83 mM) in DMF-d 7 was added a stock solution of each of the respective substrates (100 mM, 3 mL per equivalent). A total of 60 eq. was added in 10 steps and the change was followed by 1 H-NMR spectroscopy. Chemical shis of PDI-hydrogens d and e, as well as the signals of the respective substrate were followed. Fitting the binding curve was done using the tool: http://supramolecular.org/ with the NMR 1 : 1 model and the Nelder-Mead method.