Ginsenosides Rd, Rb1, Rb2, Rg1, Rg2, Rg3, and Rh2 were obtained from Tai-He Biopharmaceutical Co. Ltd. (Guangzhou, China), and relative information was summarized in Supplementary Table 1 . Stock solutions of ginsenosides were prepared in saline containing 10% propanediol (v/v). 4-AP, AP-5, bicuculline, CNQX, FK506, glycine, Gö6983, nifedipine, NMDA, and NVP-AAM077 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ifenprodil and TTX were obtained from Tocris Bioscience (Ellisville. Mo, UK). CK59, DAPK inhibitor (DAPKi, (4Z)-4-(3-Pyridylmethylene)-2-styryl-oxazol-5-one), and PP2 were from Merck (Darmstadt, Germany). Cyclosporin A (CsA) was from Abcam (Cambridge, UK). Stock solutions of 4-AP, AP-5, bicuculline, glycine, Ifenprodil, NMDA, NVP-AAM077, and TTX were prepared in distilled water, and those of CK59, CNQX, CsA, DAPKi, FK506, Gö6983, nifedipine, and PP2 were prepared in DMSO. All these drugs were diluted to their final concentrations in the corresponding extracellular solution just before each experiment. The final concentration of DMSO was maintained at ≤0.1%.
Cyclosporin a csa
Manufactured by Abcam
Sourced in United Kingdom
Cyclosporin A (CsA) is a cyclic peptide that functions as an immunosuppressant drug. It is commonly used in research to inhibit the activity of calcineurin, a protein phosphatase involved in the activation of T cells.
Automatically generated - may contain errors
Lab products found in correlation
Glycine, by Merck Group (1 mentions)
Nifedipine, by Merck Group (1 mentions)
Fk506, by Merck Group (1 mentions)
Bicuculline, by Merck Group (1 mentions)
G 6983, by Merck Group (1 mentions)
Ifenprodil, by Bio-Techne (1 mentions)
Nvp aam077, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Azathioprine, by MedChemExpress (1 mentions)
Everolimus, by Merck Group (1 mentions)
Tofacitinib, by MedChemExpress (1 mentions)
Sulfasalazine, by MedChemExpress (1 mentions)
Methotrexate, by MedChemExpress (1 mentions)
Rapamycin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using cyclosporin a csa
1
Pharmacological Reagents for Neuroscience Research
2
Immunosuppressant Effects on Lung Cell Viability
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6‐mercaptopurine, methotrexate, tofacitinib, budesonide, 5‐aminosalicylic acid (5‐ASA), sulfasalazine, azathioprine and filgotinib were purchased from MedChem Express (USA). Cyclosporin A (CsA) and tacrolimus (FK506) were purchased from Abcam. Everolimus, dexamethasone (Dex), prednisolone (Pred), 6‐TG and MPA were purchased from Sigma. Rapamycin was purchased from Santa Cruz Biotech. All the reagents were dissolved in dimethylsulfoxide (DMSO, Sigma) except MPA, which was dissolved in methanol. The effects of these immunosuppressants on lung cell line viability were determined by MTT assay (Figure S1 ).
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