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Rabbit polyclonal anti kar2 y 115

Manufactured by Santa Cruz Biotechnology
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Rabbit polyclonal anti-Kar2 (y-115) is an antibody product used for research purposes. It is raised in rabbits and targets the Kar2 protein. This antibody can be used to detect and study the Kar2 protein in various experimental applications.

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Lab products found in correlation

Irdye 680 goat anti rabbit igg, by LI COR (2 mentions) Mouse monoclonal anti myc, by Abcam (2 mentions) Hrp conjugated mouse monoclonal anti flag m2, by Merck Group (2 mentions) Mouse monoclonal anti mbp, by New England Biolabs (2 mentions) Irdye 800cw goat anti mouse igg, by LI COR (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions)

Close spelling variants of this product

Anti-Kar2 Polyclonal rabbit Rabbit anti-TM

2 protocols using rabbit polyclonal anti kar2 y 115

1

Detecting Protein Interactions by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Denaturing whole-cell extracts were prepared in 10% trichloroacetic acid with agitation in the presence of glass beads. Precipitated proteins were solubilized in Laemmli sample buffer and appropriate amounts of protein were separated by SDS-PAGE and analyzed by western blotting. Antibodies were mouse monoclonal anti-myc (1/2000, Abcam), rabbit polyclonal anti-Kar2 (y-115) (1/2000, Santa Cruz), HRP-conjugated mouse monoclonal anti-Flag M2 (1:2000, Sigma), mouse monoclonal anti-MBP (1:2000, NEB). Secondary antibodies were used at 1/5000: IRDye 800CW goat anti-mouse IgG, IRDye 680 goat anti-rabbit IgG. Western blots were revealed using the Li-COR Bioscience Odyssey infrared imaging system.
Claeys Bouuaert C., Pu S., Wang J., Oger C., Daccache D., Xie W., Patel D.J, & Keeney S. (2021). DNA-driven condensation assembles the meiotic DNA break machinery. Nature, 592(7852), 144-149.
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2

Detecting Protein Interactions by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Denaturing whole-cell extracts were prepared in 10% trichloroacetic acid with agitation in the presence of glass beads. Precipitated proteins were solubilized in Laemmli sample buffer and appropriate amounts of protein were separated by SDS-PAGE and analyzed by western blotting. Antibodies were mouse monoclonal anti-myc (1/2000, Abcam), rabbit polyclonal anti-Kar2 (y-115) (1/2000, Santa Cruz), HRP-conjugated mouse monoclonal anti-Flag M2 (1:2000, Sigma), mouse monoclonal anti-MBP (1:2000, NEB). Secondary antibodies were used at 1/5000: IRDye 800CW goat anti-mouse IgG, IRDye 680 goat anti-rabbit IgG. Western blots were revealed using the Li-COR Bioscience Odyssey infrared imaging system.
Claeys Bouuaert C., Pu S., Wang J., Oger C., Daccache D., Xie W., Patel D.J, & Keeney S. (2021). DNA-driven condensation assembles the meiotic DNA break machinery. Nature, 592(7852), 144-149.
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