HSPCs were harvested from femurs and identified with a biotinylated hematopoietic lineage kit (B220, CD3, Gr-1, CD11b and Ter119; eBioscience) and a panel of antibodies against specific HSPC surface markers (details in Supplemental Material ). BrdU incorporation in thymi and bone marrow from 6–8 week old mice 1–3 days after a single 1.75 Gy dose of IR was performed according to manufacturer’s instructions (BD Biosciences) four hours after intraperitoneal injection (1mg). Cells were incubated with FITC-Annexin-V and 7-AAD or Caspase 3/7 detection reagent according to the manufacturer (BD Biosciences or ThermoFisher, respectively). All samples were evaluated (blinded) on a FACScalibur (BD Biosciences) and analyzed using FlowJo.
Fitc annexin 5 and 7 aad or caspase 3 7 detection reagent
Manufactured by BD
FITC-Annexin-V and 7-AAD or Caspase 3/7 detection reagent is a laboratory tool used to detect and analyze apoptosis, a form of programmed cell death. The reagent contains FITC-conjugated Annexin-V, which binds to phosphatidylserine, and 7-AAD, a DNA-binding dye. This combination allows for the identification of cells undergoing early and late stages of apoptosis.
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Lab products found in correlation
2 protocols using fitc annexin 5 and 7 aad or caspase 3 7 detection reagent
1
Analyzing HSPCs after Ionizing Radiation
2
Analyzing HSPCs after Ionizing Radiation
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HSPCs were harvested from femurs and identified with a biotinylated hematopoietic lineage kit (B220, CD3, Gr-1, CD11b and Ter119; eBioscience) and a panel of antibodies against specific HSPC surface markers (details in Supplemental Material ). BrdU incorporation in thymi and bone marrow from 6–8 week old mice 1–3 days after a single 1.75 Gy dose of IR was performed according to manufacturer’s instructions (BD Biosciences) four hours after intraperitoneal injection (1mg). Cells were incubated with FITC-Annexin-V and 7-AAD or Caspase 3/7 detection reagent according to the manufacturer (BD Biosciences or ThermoFisher, respectively). All samples were evaluated (blinded) on a FACScalibur (BD Biosciences) and analyzed using FlowJo.
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