Division of the cells was into the following groups, in which introduction of cells was with 5 μmol/L Rop and/or transfection with the corresponding plasmids except for the mimic-NC, the miR-21-5p-mimic, and the Control. Treatment of cells in the control was with normal saline. There were the Rop, the Rop + mimic-negative control (NC) (mimic-NC), the Rop + miR-21-5p-mimic (miR-21-5p-mimic), the Rop + oe-NC/KANSL2 (oe-NC/KANSL2), the Rop + miR-21-5p-inhibitor + si-NC/KANSL2 (miR-21-5p-inhibitor and si-NC/KANSL2), the mimic-NC (mimic-NC) and the miR-21-5p-mimic (miR-21-5p). Purchase of Rop was implemented (siGBMa, Shanghai, China). Synthesis of miR-21-5p-mimic/inhibitor and the corresponding NCs was conducted (Sangon Biotechnology, Shanghai, China). Purchase of siRNA targeting KANSL2, elevated plasmid, and corresponding NC was conducted (Genepharma, Shanghai, China).
Mir 21 5p mimic
Manufactured by Sangon
Sourced in China
MiR-21-5p-mimic is a synthetic RNA molecule designed to mimic the function of the endogenous microRNA, miR-21-5p. Its core function is to regulate gene expression by binding to target messenger RNAs, typically leading to their translational repression or degradation.
Automatically generated - may contain errors
2 protocols using mir 21 5p mimic
1
Rop and miR-21-5p Modulation in Cell Division
2
Modulating miR-21-5p in Aβ-Induced hNSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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StemProÒ human neural stem cells (hNSCs) (H9-derived) were purchased from ThermoFisher (USA). The medium complete was used for the optimal growth and expansion of hNSCs and to maintain the undifferentiated state of hNSCs as previously described (Mcc et al. 2021 ). The cells were divided into four groups, blank control, Aβ group with 5 μM Aβ1-42 (cat: P2000022, PLlabs, Canada) treatment for 24 h, AuNPs (Yaphank, USA) group with 10 ppm AuNPs treatment for 24 h, 10 ppm AuNPs were added into Aβ group for another 24 h. hNSCs were transfected with miR-21-5p inhibitor, inhibitor-NC, miR-21-5p mimic and mimic-NC using Lipofectamine 2000 (cat: no. 11668019, Invitrogen, USA). inhibitor-NC, inhibitor, miR-21-5p mimic and mimic-NC were purchased from Sangon Biotech (Shanghai). The sequence of NC and inhibitor were as follows: miR-21-5p inhibitor, 5'-UCAACAUCAGUCUGAUAAGCUA-3'; inhibitor negative control, 5'-CAGUACUUUUGUGUAGUACAAA-3'; miR-21-5p mimic, 5'-AACAUCAGUCUGAUAAGCUAUU-3'; mimic-NC, 5'-UUGUACUACACAAAAGUACUG-3'.
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