Log In Sign up
The largest database of trusted experimental protocols

Mouse ccl21 6ckine duoset elisa

Manufactured by R&D Systems
Visit Supplier

The Mouse CCL21/6Ckine DuoSet ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse CCL21/6Ckine in cell culture supernates, serum, and plasma.

Automatically generated - may contain errors

Lab products found in correlation

Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (2 mentions) T per, by Thermo Fisher Scientific (2 mentions) Halt protease and phosphatase inhibitor mixture, by Thermo Fisher Scientific (2 mentions) Pierce bca protein assay, by Thermo Fisher Scientific (2 mentions) Mouse ccl19 mip 3 beta duoset elisa, by R&D Systems (2 mentions)

Spelling variants of this product

DuoSet ELISA (635 citations) ELISAs (144 citations) CCL21 (67 citations) DuoSets (49 citations) Mouse CCL21 (3 citations) DuoSet Mouse (2 citations)

2 protocols using mouse ccl21 6ckine duoset elisa

1

Spleen Chemokine Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were harvested, flash frozen, and stored at −80°C. Spleens were homogenized in 1 ml of T-PER (Thermo Fisher) with 5 μM EDTA (Invitrogen) and 1× Halt protease and phosphatase inhibitor mixture (Thermo Fisher). Homogenates were centrifuged at 1000 × g for 10 min, and supernatants were aliquoted and frozen at −80°C until analysis. CCL21 was measured using mouse CCL21/6Ckine DuoSet ELISA (R&D Systems). CCL19 was measured using mouse CCL19/MIP-3 beta DuoSet ELISA (R&D Systems). Chemokine concentrations were normalized to total protein concentration, determined using Pierce BCA Protein Assay (Thermo Fisher).
Masters A.R., Jellison E.R., Puddington L., Khanna K.M, & Haynes L. (2018). Attrition of T Cell Zone Fibroblastic Reticular Cell Number and Function in Aged Spleens. ImmunoHorizons, 2(5), 155-163.
+ Open protocol
+ Expand
2

Spleen Chemokine Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were harvested, flash frozen, and stored at −80°C. Spleens were homogenized in 1 ml of T-PER (Thermo Fisher) with 5 μM EDTA (Invitrogen) and 1× Halt protease and phosphatase inhibitor mixture (Thermo Fisher). Homogenates were centrifuged at 1000 × g for 10 min, and supernatants were aliquoted and frozen at −80°C until analysis. CCL21 was measured using mouse CCL21/6Ckine DuoSet ELISA (R&D Systems). CCL19 was measured using mouse CCL19/MIP-3 beta DuoSet ELISA (R&D Systems). Chemokine concentrations were normalized to total protein concentration, determined using Pierce BCA Protein Assay (Thermo Fisher).
Masters A.R., Jellison E.R., Puddington L., Khanna K.M, & Haynes L. (2018). Attrition of T Cell Zone Fibroblastic Reticular Cell Number and Function in Aged Spleens. ImmunoHorizons, 2(5), 155-163.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!