Confocal microscopy analysis of popliteal LNs was performed as previously described (37 (link)). The following primary Abs were used for staining: rat anti-B220 (RA3-6B2), rat anti-CD169 (AbD Serotec), rabbit anti-GFP (Invitrogen), rat anti-LCMV Nucleoprotein (VL-4, Bioxcell), rat anti-TCRβ (H57-597, BioLegend). The following secondary Abs were used for staining: Alexa Fluor 488-, 514-, 568- or 647-conjugated goat anti-rabbit or anti-rat IgG (Life Technologies), Alexa Fluor 647-conjugated chicken anti-rat IgG (Life Technologies). Images were acquired on an inverted Leica microscope (SP8, Leica Microsystems) with a motorized stage for tiled imaging. To minimize fluorophore spectral spillover, we used the Leica sequential laser excitation and detection modality. B cell follicles, interfollicular areas (IFA) and T cell areas were defined based on the positioning of naïve B cells and, in certain experiments, by B220 and TCRβ stainings, as described (44 (link)).
Rat anti cd169
Manufactured by Bio-Rad
The Rat anti-CD169 is a monoclonal antibody that binds to the CD169 antigen, also known as Sialoadhesin or Siglec-1. CD169 is a cell surface glycoprotein primarily expressed on macrophages. This antibody can be used for the identification and characterization of CD169-positive cells in various applications.
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Lab products found in correlation
2 protocols using rat anti cd169
1
Confocal Microscopy Analysis of Popliteal Lymph Nodes
2
Immunohistochemical Staining of Rat Tissues
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The antibodies used were rat anti‐CD169 (AbD Serotec, MCA‐884; 1:200), rabbit anti‐iba1 (Waco, 019‐19741; 1:200) and rabbit anti‐HO‐1 (Abcam, ab13243; 1:250). The secondary antibodies were biotin conj. goat anti‐rat (Amersham, RPN 1005; 1:1500), followed by Vectastain ABC reagent Vector (PK‐7100) and DAB (DakoCytomation), using the companies' recommendations. For rabbit antibodies, EnVision (DakoCytomation) was used. For double‐staining RH RedX‐conjugated goat anti‐rat (Jackson, 112‐295‐102) and Dylight 488‐conjugated goat anti‐rabbit (Jackson, 111‐485.144) were used. Specimens incubated with rat IgG2a (AbD Serotec), irrelevant rabbit antibodies or no primary antibodies served as controls.
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