Milliplex magnetic bead panel kits

Manufactured by Thermo Fisher Scientific

Sourced in France

Milliplex magnetic bead panel kits are a multiplex assay system designed for the simultaneous quantification of multiple analytes in a single sample. The kits utilize color-coded magnetic beads coated with capture antibodies specific to the target analytes. The assay protocol involves incubating the sample with the magnetic beads, followed by detection antibodies and reporter molecules. The analytes are then measured using a flow cytometry-based instrument.

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Lab products found in correlation

Milliplex analyst software, by Merck Group (2 mentions) Bms625, by Thermo Fisher Scientific (1 mentions)

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Magnetic beads (264 citations)

2 protocols using milliplex magnetic bead panel kits

Multiplex Cytokine Profiling in Plasma

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Plasma levels of pro-inflammatory cytokines (interleukin 1β (IL-1β) and Tumor Necrosis Factor α (TNFα)), anti-inflammatory cytokines (interleukin 4 (IL-4) and interleukin 10 (IL-10)), monocyte/macrophage chemokines (Macrophage Inflammatory Protein (MIP-1α), Monocyte Chemoattractant Protein (MCP-1)) and osteoprotegerin (OPG) were measured by using Milliplex magnetic bead panel kits (eBioscience, Vienne, Austria) that were analyzed using a Luminex MAGPIX system (Luminex Corporation; Houston, TX) and Milliplex Analyst software (Millipore; St. Charles, MO). The limits of detection provided by the manufacturer for IL-1β, TNFα, IL-4, IL-10, MIP-1α, MCP-1 and OPG were (pg/mL) 13, 3.78, 0.82, 8.59, 2.32, 18 and 1 respectively.

Totoson P., Maguin-Gaté K., Nappey M., Wendling D, & Demougeot C. (2016). Endothelial Dysfunction in Rheumatoid Arthritis: Mechanistic Insights and Correlation with Circulating Markers of Systemic Inflammation. PLoS ONE, 11(1), e0146744.

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Cytokine Levels in Inflammatory Models

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Levels of TNF-α, IL-17A and IL-1β levels were measured using Milliplex magnetic bead panel kits (eBioscience, Vienna, Austria) that were analyzed using a Luminex MAGPIX system (Luminex Corporation; Houston, TX) and Milliplex Analyst software (Millipore; St. Charles, MO). The limits of detection provided by the manufacturer for IL-17A, IL-1β and TNFα were 3.32 pg/mL, 13 pg/mL and 3.78 pg/mL respectively. IL-6 levels were measured using ELISA technique (rat IL6 platinum ELISA, BMS625^®, eBioscience, Vienna, Austria—detection limit: 12 pg/ml).

Guillot X., Martin H., Seguin-Py S., Maguin-Gaté K., Moretto J., Totoson P., Wendling D., Demougeot C, & Tordi N. (2017). Local cryotherapy improves adjuvant-induced arthritis through down-regulation of IL-6 / IL-17 pathway but independently of TNFα. PLoS ONE, 12(7), e0178668.

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