Pe cy7 conjugated cd3
PE/Cy7‐conjugated CD3 is a fluorescent-conjugated antibody that recognizes the CD3 protein expressed on the surface of T cells. It is a reagent used for the identification and analysis of T cells in flow cytometry applications.
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2 protocols using pe cy7 conjugated cd3
Stimulation and Immunophenotyping of PBMCs
Analyzing Virus-Induced Apoptosis and T-Cell Subsets
To analyze the levels of CD3+CD8+ T cells, 20 µL of peripheral blood sample from each group was preincubated with anti-FcγRIII/II (Fc block) (Anti-Mouse CD16/32; 553141). After 30 min of incubation, the following fluorochrome-labelled antibodies: PeCY-7-conjugated CD3 (Clone: 145-2C11; Biolegend), PE-conjugated CD4 (Clone: GK 1.5; Biolegend) and APC-conjugated CD8 (Clone: 53-6.7; Biolegend), were added to the samples for a further 30 min of incubation at room temperature. Afterward, ammonium-chloride-potassium (ACK) lysing buffer was used to lyse red blood cells in the blood samples. Finally, samples were resuspended in 500 µL of staining buffer and analyzed by a flow cytometer (LSRII Fortessa; BD, San Jose, CA).
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