Sybr green

Manufactured by Novoprotein

Sourced in China

SYBR Green is a fluorescent dye used in molecular biology for the detection and quantification of DNA in real-time PCR experiments. It binds to double-stranded DNA, emitting a fluorescent signal that can be measured and used to track the amplification of target DNA sequences.

Automatically generated - may contain errors

Lab products found in correlation

Reverse transcription kit, by Takara Bio (2 mentions) Trizol kit, by Thermo Fisher Scientific (1 mentions) Cdna synthesis kit, by Novoprotein (1 mentions)

3 protocols using sybr green

Quantitative Analysis of Mitochondrial Genes

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Total RNAs from treated cells were extracted using RNeasy Mini Kit (Yishan, Shanghai, China) according to the manufacturer’s suggestions. One microgram of total RNA was subjected to reverse transcription using the Reverse Transcription Kit (Takara, Dalian, China). The RT-qPCR primers for the genes detected were shown in Table 3. Real-time PCR assays were then performed with SYBR Green (Novoprotein, Shanghai, China) in a Rocgene Archimed X6 Real-Time PCR Detection System. 18 S were used as an internal control. The fold changes of respective genes were calculated with the 2^-ΔΔCt method.Table 3

Primers used for RT-qPCR reaction.

Gene	Forward	Reverse
ZNF281	TCTTCACCTCTCCACAACCAC	TGTAGCATCCAAAGCAGACAA
18 S	GTAACCCGTTGAACCCCATT	CCATCCAATC GGTAGTAGCG
TFAM	CCAAAAAGACCTCGTTCAGCTT	CTTCAGCTTTTCCTGCGGTG
PGC-1α	GCTTTCTGGGTGGACTCAAGT	GAGGGCAATCCGTCTTCATCC
NRF1	CTTATCCAGGTTGGTACGGGG	CTCACCTCCCTGTAACGTGG
TFB1M	GTCCTGGGTGAGGTAGGGT	TTTCCTTACAATCTTATCTGTCAGC
TFB2M	GGAGTGCAATCCAGGTCC	GAGCCCTCGAGAAGACATAGC
MRPL12	CTCAACGAGCTCCTGAAGAAAA	ACGGTGAAATGTGTCCGTTCT
NDUFS3	GTTCTGTTGCTGCCGGTGA	CAGGACACCTGAACTTGTTGGA
SDHB	TATGCAGGCCTATCGCTGGA	ATGGTGTGGCAGCGGTATAG
BCS1L	GTCACGGCGGTTTTCGTAAC	TCCAGCCCCAAAGTAGGGAT
COX6B1	CGGGGTGCCTTTAGGATTCA	TTCTGACAGCGGTGGAAGTC
ATPAF2	GGAGGAGGAGTACCAGATCCA	GCTTGTGCTTGACTGTGGTG
MT-CO2	CCTGCGACTCCTTGACGTTG	AGCGGTGAAAGTGGTTTGGTT

Zhao Q., Zhang C., Zhang X., Wang S., Guo T., Yin Y., Zhang H., Li Z., Si Y., Lu Y., Cheng S, & Ding W. (2023). ZNF281 inhibits mitochondrial biogenesis to facilitate metastasis of hepatocellular carcinoma. Cell Death Discovery, 9, 396.

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Quantitative Expression Analysis of Target Genes

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Total RNA was isolated from the cells and tissues by a TRIZOL kit (Invitrogen) based on the manufacturer's protocol. RNA was quantified and then reverse‐transcribed into cDNA by a cDNA synthesis kit (Novoprotein). qRT‐PCR was conducted by SYBR Green (Novoprotein) in an ABI 7000 thermal cycler. RNA expression was analyzed using the 2^−ΔΔCT method. GAPDH or U6 was used as the endogenous control for normalization. The primer sequences were synthesized by Tsingke Biotechnology. The sequence of the primers is outlined in Table S2.

Wang J., Chen Y., Wang Q., Xu H., Jiang Q., Wang M., Li S., Chen Y., Wu C., Yu P., Xiao Z., Chen W, & Lan Q. (2022). LncRNA SPRY4‐IT1 facilitates cell proliferation and angiogenesis of glioma via the miR‐101‐3p/EZH2/VEGFA signaling axis. Cancer Medicine, 12(6), 7309-7326.

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Quantifying Epithelial-Mesenchymal Transition Markers

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Total RNAs were extracted using an RNeasy Mini Kit (Yishan, Shanghai, China) according to the manufacturer’s protocol. One microgram of total RNA was subjected to reverse transcription assays using the Reverse Transcription Kit (Takara, Dalian, China). The RT-qPCR primers for the detected genes are shown in Table 2. The qPCR assays were performed using SYBR Green (Novoprotein, Shanghai, China) with a Rocgene Archimed X6 Real-Time PCR Detection System. 18S RNAs were used as internal control. The fold changes were calculated with the 2^−ΔΔCt method.Table 2

Primers Used for RT-qPCR Reaction

Gene	Forward (5’-3’)	Reverse (5’-3’)
ZNF281	TCTTCACCTCTCCACAACCAC	TGTAGCATCCAAAGCAGACAA
ANXA10	GCTGGCCTCATGTACCCAC	CAAGCAGTAGGCTTCTCGC
18S	GTAACCCGTTGAACCCCATT	CCATCCAATC GGTAGTAGCG
E-cadherin	CGAGAGCTACACGTTCACGG	GGGTGTCGAGGGAAAAATAGG
N-cadherin	TCAGGCGTCTGTAGAGGCTT	GGAGAAGGTCCGAGCACA
SNAIL1	GCACATCCGAAGCCACAC	ATGCACATCCTTCGATAAGACTG
SLUG	TGGTTGCTTCAAGGACACAT	GTTGCAGTGAGGGCAAGAA
VIMENTIN	TACAGGAAGCTGCTGGAAGG	ACCAGAGGGAGTGAATCCAG
ZEB1	TCAAAAGGAAGTCAATGGACAA	GTGCAGGAGGGACCTCTTTA
TWIST	AGCTACGCCTTCTCGGTCT	CCTTCTCTGG AAACAATGACATC
HDAC1	ATCGGTTAGGTTGCTTCA	TCATTCGTGTTCTGGTTAGTC
MTA1	ACGCAACCCTGTCAGTCTG	GGGCAGGTCCACCATTTCC

Zhang X., Zhang C., Zhao Q., Wang S., Wang L., Si Y., Su Q., Cheng S, & Ding W. (2023). Inhibition of Annexin A10 Contributes to ZNF281 Mediated Aggressiveness of Hepatocellular Carcinoma. Journal of Hepatocellular Carcinoma, 10, 553-571.

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