Flow cytometry analysis of heart, blood, and spleen MNCs was performed using the directly conjugated monoclonal mouse antibodies anti‐CD115 Alexa488, anti‐CD11b PerCP/Cy5.5, anti‐Ly6C Brilliant Violet 421, anti‐CXC3CR1 PE and anti‐CCR2 Alexa647 antibody. Spleen MNCs were stained with anti‐mouse CD11c PercP Cy5.5, anti‐mouse F4/80 APC, anti‐mouse vascular cell adhesion molecule (VCAM) PE, anti‐mouse GM‐CSF PE (Biolegend, San Diego, CA), anti‐mouse CD4 vio Blue and anti‐mouse IL‐3 APC (Miltenyi, Bergisch Gladbach, Germany). Surface staining was performed according to the manufacturer's instructions. Sample analysis was performed on a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) and flow cytometry data were analyzed with FlowJo 8.7. software (FlowJo, LLC, RO). Supporting Information Figure 1 shows representative flow cytometry analysis of monocytes subsets according to Yang et al. 6 .
Anti mouse cd11c percp cy5
Manufactured by BioLegend
Sourced in United States
Anti-mouse CD11c PerCP-Cy5.5 is a fluorophore-conjugated antibody that binds to the CD11c cell surface marker, which is expressed on dendritic cells and certain subsets of macrophages and monocytes in mice.
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Lab products found in correlation
2 protocols using anti mouse cd11c percp cy5
1
Multicolor Flow Cytometry Analysis of Immune Cells
2
Phenotypic Analysis of Stimulated BMDCs
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The prepared BMDCs (1 × 106 cells/mL/well) were cultured with the compounds for 24 h at 37 °C and stained with anti-mouse CD11c-PerCP/Cy5.5, CD40-APC, CD80-FITC and CD86-BV650 antibodies (BioLegend, San Diego, CA, USA) at room temperature for 30 min in the dark. The cells were analyzed using an Attune NxT Flow Cytometer (Invitrogen, Waltham, MA, USA).
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