Pro analysis grade orthophosphoric acid

The reagents used in the chromatographic separation of mycotoxins and their extraction from feed samples were: acetonitrile (ACN) and methanol (MeOH), both of gradient grade and purchased from Honeywell Riedel-de Haën (Muskegon, MI, USA); chloroform and water, also liquid chromatography (LC) gradient grade, provided by Fisher Scientific (Bishop Meadow, UK); and pro-analysis grade orthophosphoric acid obtained from Panreac (Barcelona, Spain). In addition, OASIS PRIME HLB SPE cartridges were obtained from Waters (Milford, MA, USA). In the case of DON and STER analysis, commercial enzyme-linked immunosorbent assay (ELISA) kits suitable for mycotoxin quantification in feed were used. For DON analysis, the RIDASCREEN^® FAST DON SC kit (Art. No. R5905 R-Biopharm AG, Darmstadt, Germany) was employed; while for STER quantification, the Creative-diagnostic Sterigmatocystin DEIANJ08 ELISA kit was selected (NY, USA). In both cases, all the reagents needed were included in the kits.

LC-gradient grade acetonitrile (ACN) and methanol (MeOH) were purchased from Honeywell Riedel-de Haën (Muskegon, MI, USA). LC-gradient grade chloroform and water were obtained from Fisher Scientific (Bishop Meadow, UK). Pro-analysis grade orthophosphoric acid was obtained from Panreac (Barcelona, Spain). OASIS PRIME HLB SPE cartridges were obtained from Waters (Milford, MA, USA).