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Phospho msk1

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Phospho-MSK1 is a lab equipment product that detects the phosphorylation of Mitogen- and Stress-activated Protein Kinase 1 (MSK1). MSK1 is a serine/threonine protein kinase that is activated by extracellular signal-regulated kinases (ERKs) and p38 mitogen-activated protein kinases (p38 MAPKs).

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Lab products found in correlation

Phospho atf2, by Cell Signaling Technology (2 mentions) Phospho creb, by Cell Signaling Technology (2 mentions) Arsenic trioxide as2o3, by Merck Group (1 mentions) Total p38α mapk, by Cell Signaling Technology (1 mentions) Basal medium eagle bme, by Merck Group (1 mentions) Protease inhibitor cocktail, by GenDEPOT (1 mentions) Calf serum, by Thermo Fisher Scientific (1 mentions) Total msk1, by Cell Signaling Technology (1 mentions) Total creb, by Cell Signaling Technology (1 mentions) Phospho p38 mapk, by Cell Signaling Technology (1 mentions) Antibodies against phospho akt473, by Cell Signaling Technology (1 mentions) Fitc conjugated anti mouse ly6g antibody, by BioLegend (1 mentions) Mpo elisa kit, by R&D Systems (1 mentions) Elisa kit for tnf α, by Thermo Fisher Scientific (1 mentions) Apc cy7, by BioLegend (1 mentions) Pe conjugated anti mouse human cd11b antibody, by BioLegend (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Phospho mkk3 6, by Cell Signaling Technology (1 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions)

Spelling variants of this product

Phospho-MSK1 (Thr 581) (3 citations)

3 protocols using phospho msk1

1

Signaling Pathway Activation Analysis

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Chemical reagents, including arsenic trioxide (As2O3) and basal medium eagle (BME) were purchased from Sigma-Aldrich (St. Louis, MO). Calf serum (CS) was from GIBCO (Grand Island, NY). The prestained protein marker and protease inhibitor cocktail were from GenDEPOT (Barker, TX). Antibodies against phospho-p38 MAPK, total p38α MAPK, phospho-MSK1, total MSK1, phospho-CREB, total CREB, phospho-ATF2 and total ATF2 were from Cell Signaling Technology, Inc. (Beverly, MA).
Kim H.G., Shi C., Bode A.M, & Dong Z. (2015). p38α MAPK is required for arsenic-induced cell transformation. Molecular carcinogenesis, 55(5), 910-917.
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2

Neutrophil Activation and Inflammatory Response

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LPS (Escherichia coli 0111:B4), TUDCA were obtained from Sigma Aldrich. Anti-FPR2 (M-73) antibody was obtained from Santa Cruz Biotechnology. Antibodies against phospho-AKT473, LAMP1, phospho-CREB, phospho-MSK1, GAPDH were purchased from Cell Signaling Technology. FITC conjugated anti-mouse Ly6G antibody, PE conjugated anti-mouse CD195(CCR5) antibody, PE/Cy7 conjugated anti-mouse Ly6C antibody, APC/Cy7 and PE conjugated anti-mouse/human CD11b antibody were from Biolegend (San Diego, CA). FPRL1 (bs-3654R) antibody was from Bioss Antibodies. Anti-citrullinated Histone H4 antibodywas fromMillipore (Billerica,MA). ELISA Kit for TNF-α, IL1-β, IL-6 and IL-10 from eBioscience respectively. MPO ELISA Kit from R&D system.
Diao N., Zhang Y., Chen K., Yuan R., Lee C., Geng S., Kowalski E., Guo W., Xiong H., Li M, & Li L. (2016). Deficiency in Toll-interacting protein (Tollip) skews inflamed yet incompetent innate leukocytes in vivo during DSS-induced septic colitis. Scientific Reports, 6, 34672.
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3

Western Blot Analysis of Signaling Pathways

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Protein from cells was dissolved in 1X SDS loading buffer and separated on SDS-PAGE gels. Subsequently, proteins were electroblotted onto PVDF membranes (Bio-Rad Laboratories), probed with primary and secondary antibodies, and detected using the enhanced chemiluminescence (ECL) system (Pierce, Rockford, IL). The primary antibodies included the following: Phospho-ERK (Thr202/Tyr204), Phospho-p38 (Thr180/Tyr182), Phospho-p65 (Ser 468), Phospho-STAT3 (Tyr705), Phospho-JAK2 (Tyr1007/Tyr1008), Phospho-JNK (Thr183/Tyr185), p38, Phospho-MKK3/6 (Ser189/Ser207), Phospho-ATF2 (Thr71), Phospho-HSP27 (Ser81), Phospho-MAPKAPK2 (Thr334), and Phospho-MSK1 (Thr581) from Cell Signaling Technology (Beverly, MA); phospho-JAK1 (Thy1022/Tyr1023) from EMD Millipore (Billerica, MA); ZIKV Envelope protein (ZENV11-S) from Alpha Diagnostic Intl. Inc (San Antonio, TX); mouse monoclonal anti-α-Tubulin from Sigma-Aldrich was used as loading control. Densitometry analysis was conducted using ImageJ software.
Zhu S., Luo H., Liu H., Ha Y., Mays E.R., Lawrence R.E., Winkelmann E., Barrett A.D., Smith S.B., Wang M., Wang T, & Zhang W. (2017). p38MAPK plays a critical role in induction of a pro-inflammatory phenotype of retinal Müller cells following Zika virus infection. Antiviral research, 145, 70-81.
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