Planarians were sacrificed by placing them in 5.7% of 12N HCL solution for 5 mins and fixing them in Carnoys solution for 2 hours on ice. After this, planarians were stored in methanol at −20°C for at least an hour and then bleached overnight in 6% H2O2 solution. Animals were rehydrated in dilutions of Methanol:PBSTx and stained as previously described (Thiruvalluvan et al., 2018 (link)). Primary antibodies: α-H3p, 1:250 (Millipore Cat# 05–817R) and caspase- 3, 1:500 (Abcam ab13847). Secondary antibodies: Goat anti-rabbit Alexa568, 1:800 (Invitrogen Cat# 11036) for H3P, HRP-conjugated goat anti-rabbit antibody (Millipore Cat# 12–348) with TSA-Alexa568 anti-HRP for caspase-3, 1:2000. For C. albicans staining and p-38 staining in the planaria whole mounts, animals were sacrificed in 10% NAC diluted in PBS. The animals were then fixed in 4% formaldehyde in 0.3% PBSTx and permeabilized with 1% SDS. Animals were then bleached in 6% H2O2 in 1xPBS. Primary antibodies: Anti-Candida, 1:500 (ThermoFisher Cat# PA1–27158) and anti-phospho p38, 1:800 (Cell Signaling Technologies CAT# 9211).
Anti candida
Manufactured by Thermo Fisher Scientific
Anti-Candida is a laboratory product designed to detect and identify Candida species, which are a group of fungi commonly found in the human body. The product provides a reliable method for the detection and identification of Candida species, which is essential for the diagnosis and management of Candida infections.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated goat anti rabbit antibody, by Merck Group (3 mentions)
α h3p, by Merck Group (3 mentions)
Goat anti rabbit alexa568, by Thermo Fisher Scientific (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Caspase 3, by Abcam (1 mentions)
Alexa 568, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti candida
1
Planarian Fixation and Staining
2
Planarian Immunohistochemistry Protocol
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Non-infected and infected planarians were sacrificed with 5.7% 12N HCL solution for 5 min and fixed using Carnoys solution for 2 h on ice. After the fixation, animals were stored in methanol at −20°C and then bleached overnight in a 6% H2O2 solution. Animals were then rehydrated in dilutions of methanol:PBSTx and stained as previously described (Ziman et al., 2020 (link)). The primary antibody used was α-H3p, 1:250 (Millipore Cat# 05–817R). The secondary antibodies used were goat anti-rabbit Alexa568, 1:800 (Invitrogen Cat# 11036) and HRP-conjugated goat anti-rabbit antibody (Millipore Cat# 12-348).
To stain C. albicans cells, infected animals were sacrificed in 10% NAC diluted in PBS. The planarians were then fixed in 4% formaldehyde in PBTx and permeabilized in 1% SDS. The animals were bleached in 3% H2O2 in 1× PBS. The primary antibody used was anti-Candida, 1:500 (Thermo Fisher Cat# PA1-27158). The secondary antibody used was goat anti-rabbit Alexa568, 1:800 (Invitrogen Cat# 11036).
To stain C. albicans cells, infected animals were sacrificed in 10% NAC diluted in PBS. The planarians were then fixed in 4% formaldehyde in PBTx and permeabilized in 1% SDS. The animals were bleached in 3% H2O2 in 1× PBS. The primary antibody used was anti-Candida, 1:500 (Thermo Fisher Cat# PA1-27158). The secondary antibody used was goat anti-rabbit Alexa568, 1:800 (Invitrogen Cat# 11036).
3
Planarian Cellular Immunofluorescence Assay
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Non-infected and infected planarians were sacrificed with 5.7% 12N HCL solution for 5 min and fixed using Carnoys solution for 2h on ice. After the fixation, animals were stored in methanol at -20°C and then bleached overnight in a 6% H 2 O 2 solution. Animals were then rehydrated in dilutions of methanol:PBSTx and stained as previously described (Ziman et al., 2020) . The primary antibody used was α-H3p, 1:250 (Millipore Cat# 05-817R). The secondary antibodies used were goat anti-rabbit Alexa568, 1:800 (Invitrogen Cat# 11036) and HRP-conjugated goat anti-rabbit antibody (Millipore Cat# 12-348).
To stain C. albicans cells, infected animals were sacrificed in 10% NAC diluted in PBS. The planarians were then fixed in 4% formaldehyde in PBTx and permeabilized in 1% SDS. The animals were bleached in 3% H 2 O 2 in 1X PBS. The primary antibody used was anti-Candida, 1:500 (ThermoFisher Cat# PA1-27158). The secondary antibody used was goat anti-rabbit Alexa568, 1:800 (Invitrogen Cat# 11036).
To stain C. albicans cells, infected animals were sacrificed in 10% NAC diluted in PBS. The planarians were then fixed in 4% formaldehyde in PBTx and permeabilized in 1% SDS. The animals were bleached in 3% H 2 O 2 in 1X PBS. The primary antibody used was anti-Candida, 1:500 (ThermoFisher Cat# PA1-27158). The secondary antibody used was goat anti-rabbit Alexa568, 1:800 (Invitrogen Cat# 11036).
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