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2 protocols using l ascorbic acid

1

Antioxidant Capacity Evaluation Protocol

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Salicylic acid, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), ABTS, ferrous sulfate heptahydrate (FeSO 4 •7H 2 O), L-ascorbic acid, cupric chloride dihydrate (CuCl 2 •2H 2 O), ammonium acetate (NH 4 Ac), 2,9-dimethyl-1,10-phenanthroline (Neocuproine, Nc), quercetin, D-(+)-glucose, butylated hydroxytoluene (BHT), butyl hydroxyanisole (BHA), tertiary butylhydroquinone (TBHQ) were purchased from Energy Chemical. 6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) and β-carotene were purchased from TCI. DPPH was purchased from Alfa Aesar. Folin & Ciocalteu's phenol reagent (FC reagent), linoleic acid, aluminium chloride hexahydrate (AlCl 3 •6H 2 O) were purchased from Xiya Reagent. Sodium carbonate (Na 2 CO 3 ), methanol, ethanol, acetone, ethyl acetate, chloroform, hexane and dimethyl sulfoxide (DMSO) were purchased from Sinopharm. High-glucose Dulbecco's modified Eagle's medium (HG-DMEM) was purchased from Hyclone. Fetal bovine serum (FBS) was purchased from Gibco. 100× penicillin-streptomycin solution was purchased from Biosharp. Cell Counting Kit-8 (CCK-8) was purchased from Beyotime. TM3 mouse leydig cells were purchased from Procell.
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2

Synthesis of Metal-Doped Titanium Sheets

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Pure titanium sheets were obtained from Jinkai Co., Ltd. (Baoji, China). Y(NO3)3•6H2O, L-ascorbic acid, and PFOS were purchased from Energy Chemical (Shanghai, China). Pb(NO3)2, SnCl4•5H2O, SbCl3, Na2SO4, C3H7OH, HNO3, H2C2O4, and HCl were provided by Sinopharm (Shanghai, China). NaF and PbO were supplied by Shenyang Chemical Reagent Factory (Shenyang, China). All chemicals used were analytical reagents and used directly without any further purification. All the solutions were prepared with deionized water.
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