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Biotinylated circsmarcc1 probe

Manufactured by RiboBio
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Sourced in China

The Biotinylated-circSMARCC1 probe is a laboratory tool designed for the detection and analysis of circular SMARTCC1 RNA molecules. It contains a biotin label that enables its use in various RNA detection and purification techniques.

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Lab products found in correlation

Oligo probe, by RiboBio (2 mentions) Piercetm magnetic rna protein pull down kit, by Thermo Fisher Scientific (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions)

2 protocols using biotinylated circsmarcc1 probe

1

Enrichment of miRNA by CircRNA Pulldown

Check if the same lab product or an alternative is used in the 5 most similar protocols
To pull down the miRNA by circRNA, biotinylated-circSMARCC1 probe (5′-CATCTTCCTCATCACAGCAC-3′) was synthesized by RiboBio (Guangzhou, China), and the oligo probe (5′-TATGTTGTTGATTTGCTGGC-3′) was used as a control. CircRNA pulldown assay was carried out using PierceTM Magnetic RNA-Protein Pull-Down Kit (No: 20164, Thermo Fisher Scientific, United States). All procedures followed the manufacturer’s instructions. Then the final RNA was extracted by TRIzol (Invitrogen, United States) and analyzed by RT qPCR.
Xie T., Fu D.J., Li Z.M., Lv D.J., Song X.L., Yu Y.Z., Wang C., Li K.J., Zhai B., Wu J., Feng N.H, & Zhao S.C. (2022). CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling. Molecular Cancer, 21, 173.
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2

Enrichment of miRNA by CircRNA Pulldown

Check if the same lab product or an alternative is used in the 5 most similar protocols
To pull down the miRNA by circRNA, biotinylated-circSMARCC1 probe (5′-CATCTTCCTCATCACAGCAC-3′) was synthesized by RiboBio (Guangzhou, China), and the oligo probe (5′-TATGTTGTTGATTTGCTGGC-3′) was used as a control. CircRNA pulldown assay was carried out using PierceTM Magnetic RNA-Protein Pull-Down Kit (No: 20164, Thermo Fisher Scientific, United States). All procedures followed the manufacturer’s instructions. Then the final RNA was extracted by TRIzol (Invitrogen, United States) and analyzed by RT qPCR.
Xie T., Fu D.J., Li Z.M., Lv D.J., Song X.L., Yu Y.Z., Wang C., Li K.J., Zhai B., Wu J., Feng N.H, & Zhao S.C. (2022). CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling. Molecular Cancer, 21, 173.
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