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Tcs sp8 imaging

Manufactured by Leica Microsystems
Sourced in Germany

The TCS-SP8 imaging system is a high-performance confocal laser scanning microscope designed for advanced imaging applications. It features a modular architecture, allowing for customization to meet specific research needs.

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2 protocols using tcs sp8 imaging

1

Microscopy Techniques Utilized

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Stereo microscopy was carried out with the Leica MZ 16F stereo microscope and the LAS AF software. Light and fluorescence microscopy was carried out with the Leica DMRB microscope equipped with a Sony Alpha 6000 camera or with the Leica DMRE microscope equipped with the Leica DFC 7000 T camera using the LAS X software. Confocal laser scanning microscopy was carried out with the Leica DM5500 and DM6000 CS Microscopes and documented with the TCS-SPE and TCS-SP8 imaging systems, respectively (Leica Microsystems, Heidelberg, Germany).
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2

Biolistic Transformation of Arabidopsis Leaves

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Expression of plasmids was carried out in rosette leaves of 2 weeks old Arabidopsis seedlings by biolistic transformation (Mathur et al., 2003 (link)) and analyzed by confocal laser scanning microscopy after 12–16 h. Confocal laser scanning microscopy was performed with the Leica DM5500 and DM6000 CS Microscopes and documented with the TCS-SPE and TCS-SP8 imaging systems, respectively (Leica Microsystems, Heidelberg, Germany).
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