Cells were resuspended in 5 μl Live/Dead solution (Invitrogen-Life Technologies) for 10 min at RT, then incubated with cell surface antibodies for 30 min at RT followed by intracellular staining for Foxp3 using the anti-mouse/rat/human FOXP3 (Biolegend) following the manufacturer's instructions. Flow cytometry analysis was performed using a LSRII (BD Bioscience) and data analyzed with FlowJo software.
Anti human cd3 alexa fluor 700
The Anti-human CD3-Alexa Fluor 700 is a fluorescently labeled antibody that binds to the CD3 protein expressed on the surface of human T cells. It is designed for use in flow cytometry applications to identify and quantify T cell populations.
Lab products found in correlation
2 protocols using anti human cd3 alexa fluor 700
Phenotypic Analysis of PBMC Subsets
Cells were resuspended in 5 μl Live/Dead solution (Invitrogen-Life Technologies) for 10 min at RT, then incubated with cell surface antibodies for 30 min at RT followed by intracellular staining for Foxp3 using the anti-mouse/rat/human FOXP3 (Biolegend) following the manufacturer's instructions. Flow cytometry analysis was performed using a LSRII (BD Bioscience) and data analyzed with FlowJo software.
Immunophenotyping of T and B Cells
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