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Anti human cd3 alexa fluor 700

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Anti-human CD3-Alexa Fluor 700 is a fluorescently labeled antibody that binds to the CD3 protein expressed on the surface of human T cells. It is designed for use in flow cytometry applications to identify and quantify T cell populations.

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2 protocols using anti human cd3 alexa fluor 700

1

Phenotypic Analysis of PBMC Subsets

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Antibodies used to detect cell surface markers of PBMCs were: anti-human CD3-Alexa Fluor 700, anti-human CD4-FITC, anti-human CD25-APC-eFluor 780, anti-human CD39-PE-Cy7 and anti-human CD56-PE from eBioscience; anti-human CD8-Pacific Blue, anti-human CXCR3 PE-Cy5 and PE-Cy5 mouse IgG1, κ isotype control from BD Bioscience.
Cells were resuspended in 5 μl Live/Dead solution (Invitrogen-Life Technologies) for 10 min at RT, then incubated with cell surface antibodies for 30 min at RT followed by intracellular staining for Foxp3 using the anti-mouse/rat/human FOXP3 (Biolegend) following the manufacturer's instructions. Flow cytometry analysis was performed using a LSRII (BD Bioscience) and data analyzed with FlowJo software.
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2

Immunophenotyping of T and B Cells

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Blood samples were washed and incubated with antihuman CD3-AlexaFluor700, CD4-eFluor450 (eBioscience, San Diego, USA), CD45RO-FITC, CCR7-PE-Cy7 (BD Biosciences, Franklin Lakes, USA), CXCR3-APC-Cy7, CCR4-PerCP-Cy5.5 and CCR6-BV605 (BioLegend, San Diego, USA) to determine CD45RO + CCR7 -CCR6 + CCR4 + CXCR3 À Th EM 17 cells and CD45RO + CCR7 À CCR6 À CCR4 À CXCR3 + Th EM 1 cells [2, 9] , or anti-human CD19-eFluor450, CD38-PE-Cy7 (eBioscience) and CD24-FITC (BD Biosciences) to determine CD24 hi CD38 hi Bregs. Samples were fixed, washed and acquired on a LSR-II (BD Biosciences). For gating strategies see Supplementary Fig. S1, available at Rheumatology online.
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