Peripheral blood samples were collected following a 12-hour fasting. Glycosylated hemoglobin (HbA1c), blood glucose, total cholesterol, and triglycerides levels were estimated by routine enzymatic methods (Spinreact). TC, LDL, HDL and TG were measured using standard enzymatic methods. HDLc and LDLc were determined after precipitation of the apo B-containing lipoproteins using Friedewald formula [27 (link)]. Glycosylated hemoglobin (HbAlc) was estimated calorimetrically by using (Biosystems, Barcelona, Spain). Serum insulin and lipoprotein a (LPa) were measured by ELISA using the Quantikine human insulin and LPa assay kits (R and D Systems, Minneapolis, MN).
Quantikine human insulin and lpa assay kits
Manufactured by R&D Systems
The Quantikine human insulin and LPa assay kits are laboratory instruments designed to quantitatively measure the levels of human insulin and lipoprotein(a) (Lp(a)) in biological samples. These kits utilize enzyme-linked immunosorbent assay (ELISA) technology to provide accurate and reproducible results.
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Lab products found in correlation
2 protocols using quantikine human insulin and lpa assay kits
1
Metabolic Biomarker Profiling Protocol
2
Fasting Metabolic Profile Analysis
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Blood samples were taken from all studied subjects after 8 H fast as follows: 2 mL blood was taken on fluoride containing tubes, another 2 mL was taken on EDTA containing tubes, and the remaining part was left for 10 min to clot and then was centrifuged at 1,000 3 g for 5 min. Another blood sample was taken after 12 H fast and on fluoride containing tubes 2 H after meal (2hpp). The serum and plasma samples were separated and stored at 2208C till the time of use.
Fasting, 2hpp blood glucose, total cholesterol, and triglycerides were measured by routine enzymatic methods (Spinreact). HDLc was determined after precipitation of the apo B-containing lipoproteins. LDLc was calculated using Friedewald formula (11) . Glycated hemoglobin (HbAlc) was estimated colorimetrically by using (Biosystems, Barcelona, Spain). Serum insulin and lipoprotein a (LPa) was measured by ELISA using the Quantikine human insulin and LPa assay kits (R and D Systems, Minneapolis, MN). HOMA-IR was calculated using the following formula: [fasting insulin (lIU/mL) 3 fasting glucose (mmol/L)]/22.5.
Fasting, 2hpp blood glucose, total cholesterol, and triglycerides were measured by routine enzymatic methods (Spinreact). HDLc was determined after precipitation of the apo B-containing lipoproteins. LDLc was calculated using Friedewald formula (11) . Glycated hemoglobin (HbAlc) was estimated colorimetrically by using (Biosystems, Barcelona, Spain). Serum insulin and lipoprotein a (LPa) was measured by ELISA using the Quantikine human insulin and LPa assay kits (R and D Systems, Minneapolis, MN). HOMA-IR was calculated using the following formula: [fasting insulin (lIU/mL) 3 fasting glucose (mmol/L)]/22.5.
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