Total proteins were separated by electrophoresis on 4–20% gradient PAGE gels (Bio‐Rad, 4561093) and transferred onto nitrocellulose membrane. The following antibodies were used: anti‐phosphoAKT (Ser473) (Cell Signaling, 9271S), anti‐AKT (Cell Signaling, 9272S), anti‐phosphoCREB (Ser133) (Millipore, aa77‐343), anti‐phosphoERK (Cell signaling, 4370S), anti‐ERK (Cell signaling, 4695S), anti‐phospho‐CTD RNA polymerase II (Ser2) (Abcam, ab5095), anti‐CTD RNA polymerase II (Abcam, ab817), anti‐N‐terminal RNA polymerase II A‐10 (Santa Cruz, sc17798), anti‐ACTININ (Abcam, ab68194), anti‐COFILIN (Abcam, ab54532), anti‐GAPDH (clone D16H11, Cell Signaling, 5174), anti‐MECP2 (Cell Signaling, 3456), anti‐U1‐70K (clone H111, Synaptic Systems, 203011), anti‐LAMIN B1 (Abcam, ab133741), anti‐hnRNPA1 (clone D21H11, Cell Signaling, 8443), and anti‐betaIII TUBULIN (Abcam, ab18207).
Gradient page gels
Manufactured by Bio-Rad
4–20% gradient PAGE gels are laboratory equipment designed for protein separation and analysis using polyacrylamide gel electrophoresis (PAGE) techniques. These gels feature a continuous gradient of polyacrylamide concentrations ranging from 4% to 20%, which allows for the separation of a wide range of protein molecular weights in a single gel.
Automatically generated - may contain errors
Lab products found in correlation
Anti cofilin, by Abcam (2 mentions)
Anti mecp2, by Cell Signaling Technology (2 mentions)
Anti n terminal rna polymerase 2 a 10, by Santa Cruz Biotechnology (2 mentions)
Anti erk, by Cell Signaling Technology (2 mentions)
Anti actinin, by Abcam (2 mentions)
Anti hnrnpa1, by Cell Signaling Technology (2 mentions)
Anti phospho erk, by Cell Signaling Technology (2 mentions)
Anti phospho creb ser133, by Merck Group (2 mentions)
Anti phospho akt ser473, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti gapdh clone d16h11, by Cell Signaling Technology (1 mentions)
Anti lamin b1, by Abcam (1 mentions)
Anti u1 70k, by Synaptic Systems (1 mentions)
Anti beta 3 tubulin, by Abcam (1 mentions)
Anti creb, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
2 protocols using gradient page gels
1
Comprehensive Protein Analysis by Western Blot
2
Comprehensive Protein Analysis Protocol
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Total proteins were separated by electrophoresis on 4%-20% gradient PAGE gels (Bio-Rad, 4561093) and transferred onto nitrocellulose membrane. The following antibodies were used: anti-phosphoCREB (Ser133) (Millipore, aa77-343), anti-CREB (clone 48H2, Cell signaling, 9197), anti-phosphoERK (Cell signaling, 4370S), anti-ERK (Cell signaling, 4695S), anti-phospho-CTD RNA polymerase II (Ser2) (Abcam, ab5095), anti-CTD RNA polymerase II (Abcam, ab817), anti-N-terminal RNA polymerase II A-10 (Santa Cruz, sc17798), anti-ACTININ (Abcam, ab68194), anti-COFILIN (Abcam, ab54532), anti-GAPDH (clone D16H11, Cell Signaling, 5174), anti-MECP2 (Cell Signaling, 3456), anti-U1-70K (clone H111, Synaptic Systems, 203011), anti-LAMIN B1 (Abcam, ab133741), anti-hnRNPA1 (clone D21H11, Cell Signaling, 8443), anti-betaIII TUBULIN (Abcam, ab18207).
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