Millipore® Direct UV-Q water was used to prepare all the solutions in this study. GLY of 99.7% purity, AMPA of 99% purity and 9-flourenylmethlylchloroformate chloride (FMOC) of 97% purity were purchased from Sigma Aldrich® (Seelze, Germany), while acetonitrile was purchased from QREC®, Malaysia. Stock solutions (500 mg L−1) of GLY and AMPA were prepared by quantitatively dissolving their appropriate quantities into Millipore® Direct UV-Q water, while 0.02 M FMOC was prepared in acetonitrile. Analytical reagent grade CaCl2 and KH2PO4 were purchased from Emsure® (Germany) while HgCl2 and Na2B4O7.10H2O were purchased from Sigma Aldrich® (India).
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