Pyr 41

Manufactured by MedChemExpress

Sourced in China

PYR-41 is a potent and selective inhibitor of the E1 ubiquitin-activating enzyme. It functions by disrupting the ubiquitin-proteasome system, which plays a crucial role in protein degradation within cells.

Automatically generated - may contain errors

Lab products found in correlation

Bafilomycin a1 bafa1, by Merck Group (1 mentions) Pr 619, by MedChemExpress (1 mentions) Sto 609, by MedChemExpress (1 mentions) Chloroquine cq, by Merck Group (1 mentions) Compound c, by MedChemExpress (1 mentions) Forskolin, by Beyotime (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Ici 118 551, by MedChemExpress (1 mentions) C3h10t1 2, by American Type Culture Collection (1 mentions)

2 protocols using pyr 41

Inhibitors of Oesophageal Carcinoma Cells

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Human oesophageal squamous cell carcinoma cell line Kyse30, Kyse450, EC1 and EC109 were cultured in DMEM (BI) medium containing 10% FBS (BI) at 37℃ with 5% CO₂. PR‐619 (a pan‐DUB inhibitor), STO‐609 (a CaMKK inhibitor), Compound C (CC) (an AMPK inhibitor) and PYR‐41 (a ubiquitin E1 inhibitor) were purchased from MedChemExpress (MCE) and dissolved in dimethyl sulfoxide (DMSO). Chloroquine (CQ) was purchased from Sigma‐Aldrich and was dissolved in phosphate‐buffered saline (PBS). Bafilomycin A1(BafA1) was purchased from Sigma‐Aldrich and dissolved in DMSO.

Wang L., Li M., Sha B., Hu X., Sun Y., Zhu M., Xu Y., Li P., Wang Y., Guo Y., Li J., Shi J., Li P., Hu T, & Chen P. (2020). Inhibition of deubiquitination by PR‐619 induces apoptosis and autophagy via ubi‐protein aggregation‐activated ER stress in oesophageal squamous cell carcinoma. Cell Proliferation, 54(1), e12919.

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Insulin Signaling in Diverse Cell Lines

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The cell lines 293T (ATCC, CRL-11268), Hep G2 (ATCC, HB-8065), C3H/10T1/2 (ATCC, CCL-226), and C2C12 (ATCC, CRL-1772) were purchased from Cell Bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences. Mouse PHs and non-PHs were prepared by collagenase perfusion (6, 28) . The differentiation of C2C12 myoblasts and C3H/10T1/2 preadipocytes was induced as previously described (29, 30) . To detect insulin signaling, cells were incubated with 100 nmol/L insulin for 20 min (29, 31, 32) . For inhibiting endogenous ubiquitination, C2C12 myotubes were incubated with 50 mmol/L PYR41 for 24 h (33) (MedChemExpress, Shanghai, China). C2C12 myotubes were incubated with forskolin (34) (100 mmol/ L, 24 h; Beyotime, Shanghai, China) or ICI 118,551 (25) (0.5, 1, and 3 mmol/L, 6 h; MedChemExpress).

Niu Y., Jiang H., Yin H., Wang F., Hu R., Hu X., Peng B., Shu Y., Li Z., Chen S, & Guo F. (2022). Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity Via Inhibiting USP33-Mediated ADRB2 Deubiquitination. Diabetes, 71(5).

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