Varian cary 5g spectrophotometer

The absorption spectra were taken on a Varian-Cary 5G spectrophotometer. The fluorescence quantum yield measurements were performed using a Varian-Cary 5G spectrophotometer and a FluoroLog-3 spectrofluorometer. Spectral measurements were carried out in air-saturated acetonitrile solutions (acetonitrile of spectrophotometric grade, water content o0.005%, Aldrich) at 20 AE 1 1C; the concentrations of the studied compounds were about 0.5-2.0 Â 10 À5 M. All measured fluorescence spectra were corrected for the nonuniformity of detector spectral sensitivity. Coumarin 481 in acetonitrile (j fl = 0.08) 51 was used as a reference for the fluorescence quantum yield measurements. The fluorescence quantum yields were calculated by eqn (1), 52
wherein j fl and j fl R are the fluorescence quantum yields of the studied solution and the standard compound, respectively; A and A R are the absorption of the studied solution and the standard respectively; S and S R are the areas underneath the curves of the fluorescence spectra of the studied solution and the standard respectively; and n and n R are the refraction indices of the solvents for the substance under study and the standard compound.

The absorption spectra were taken on a Varian-Cary 5G spectrophotometer. The fluorescence quantum yield measurements were performed using a Varian-Cary 5G spectrophotometer and a FluoroMax-3 spectrofluorimeter. Spectral measurements were carried out in air-saturated acetonitrile solutions (acetonitrile of spectrophotometric grade, water content o0.005%, Aldrich) at 20 AE 1 1C; the concentrations of the studied compounds were about 0.5-2.0 Â 10 À5 M. All measured fluorescence spectra were corrected for the nonuniformity of detector spectral sensitivity. Coumarin 481 in acetonitrile (j fl = 0.08) 37 was used as a reference for the fluorescence quantum yield measurements. The fluorescence quantum yields were calculated using eqn (1): 38
wherein j fl i and j fl 0 are the fluorescence quantum yields of the studied solution and the standard compound, respectively; A i and A 0 are the absorptions of the studied solution and the standard, respectively; S i and S 0 are the areas underneath the curves of the fluorescence spectra of the studied solution and the standard, respectively; n i and n 0 are the refraction indices of the solvents for the substance under study and the standard compound.

The absorption spectra were taken on a Varian-Cary 5G spectrophotometer. The fluorescence quantum yield measurements were performed using a Varian-Cary 5G spectrophotometer and a FluoroMax-3 spectrofluorometer. Spectral measurements were carried out in air-saturated acetonitrile solutions (acetonitrile of spectrophotometric grade, water content o0.005%, Aldrich) at 20 AE 1 1C; the concentrations of the studied compounds were about 0.5-2.0 Â 10 À5 M. All measured fluorescence spectra were corrected for nonuniformity of detector spectral sensitivity. Coumarin 481 in acetonitrile (j fl = 0.08) 45 was used as a reference for the fluorescence quantum yield measurements. The fluorescence quantum yields were calculated using 46
where j fl i and j fl 0 are the fluorescence quantum yields of the studied solution and the standard compound, respectively; A i and A 0 are the absorptions of the studied solution and the standard, respectively; S i and S 0 are the areas underneath the curves of the fluorescence spectra of the studied solution and the standard, respectively; and n i and n 0 are the refraction indices of the solvents for the substance under study and the standard compound (n i = n 0 = 1.342, acetonitrile).