Anaerobic atmosphere kit

Samples of 10 g of fish muscle were dissected aseptically from refrigerated fish specimens, mixed with 90 mL of 0.1% peptone water (Merck, Darmstadt, Germany) and homogenised in sterilised stomacher bags (AES, Combourg, France) as previously described (Ben-Gigirey et al., 1998; Ben-Gigirey et al., 1999) . In all of the cases, serial dilutions from the microbial extracts were prepared in 0.1% peptone water.
Total aerobes were investigated by surface inoculation on plate count agar (PCA, Oxoid Ltd., London, UK) after incubation at 30ºC for 48 h. The anaerobe counts were also determined in PCA at 30±0.5ºC, except that an anaerobic atmosphere kit (Oxoid Ltd.) was placed together with the plates inside the anaerobiosis jar.
Psychrotrophs were also investigated in PCA, being the incubation carried out at 7-8ºC for 7 days. Enterobacteriaceae were investigated via pour plating using Violet Red Bile Agar (VRBA) (Merck, Darmstadt, Germany) after incubation at 37±0.5ºC for 24 h. In all of the cases, bacterial counts were transformed into log CFU g -1 muscle before undergoing statistical analysis. All of the analyses were conducted in triplicate.

Muscle samples (10 g) were dissected aseptically from chilled lobster krill specimens, mixed with 90 ml of 0.1 % peptone water (Merck, Darmstadt, Germany), and homogenised in sterilized stomacher bags (AES, Combourg, France) as previously described (Ben-Gigirey et al., 1998 , 1999) . In all cases, serial dilutions of the microbial extracts were prepared in 0.1 % peptone water.
Total aerobes were determined by surface inoculation on plate count agar (PCA, Oxoid Ltd., London, UK), after incubation at 30ºC for 48 h. The anaerobe counts were also determined on PCA at 30ºC, except that an anaerobic atmosphere kit (Oxoid) was placed together with the plates inside an anaerobic jar. Psychrotrophes were also determined on PCA but incubation was carried out at 7-8ºC for 7 d. Enterobacteriaceae were determined by pour plating using Violet Red Bile Agar (VRBA) (Merck, Darmstadt, Germany) after incubation at 37ºC for 24 h. Microorganisms exhibiting a proteolytic or lipolytic phenotype were determined on casein-agar medium or tributyrine-agar, respectively, after incubation at 30ºC for 48 h, as previously described (Ben-Gigirey et al., 2000) .
In all cases, bacterial counts were transformed into log CFU g -1 muscle before undergoing statistical analysis. All microbiological analyses were done in triplicate.