Kgm12800nh 500

HEK293T, HK-2, ACHN, 786-O, Caki-1, KETR3, and OS-RC2 cell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). 786-O, Caki-1, KETR3, and OS-RC2 cells were cultured in RPMI-1640 medium (KGM31800NH-500, KeyGEN BioTECH) supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin and incubated in a 37^◦C humidified incubator with 5% CO₂. ACHN cells were cultured in MEM (KGM41500N-500, KeyGEN BioTECH), HEK293T, and HK-2 cells were cultured in DMEM (KGM12800NH-500, KeyGEN BioTECH), and the other cell culture conditions were the same as those described above.

Human umbilical vein endothelial cells (Huvec) and human embryonic kidney 293T (HEK293T) cells were obtained from the Cell Bank of China Science Academy (Shanghai, China). The U87 cells, U373 cells and LN229 cells were purchased from American Type Culture Collection.U87, U373, LN229, HUVEC, and HEK293T cell lines were maintained in DMEM (KGM12800NH-500, Keygen Biotech, China) supplemented with 10% fetal bovine serum (BC-SE-FBS01, Biochannel, China) at 37 °C, in 5% CO₂ humid atmosphere.
All plasmids were purchased from GenePharma Technology (Shanghai, China). Lipofectamine 2000 (Life Technologies, Carlsbad, CA, USA) was used for plasmid transfection according to the instructions of the product side.