Anti-α-tubulin monoclonal antibody was purchased from Sigma-Aldrich. Anti-eIF4γ, anti-BiP/Grp78, anti-PKCδ and anti-G3BP1 monoclonal antibodies were from BD Bioscience; anti-Atg5, anti-Atg16L1, anti-eIF2α, anti-phospho-eIF2α, anti-cleaved caspase7 (cCas7), anti-PERK, anti-cleaved poly(ADP-ribose) polymerase (cPARP), anti-PKD1, anti-phospho-(Ser/Thr) PKD substrates and anti-TIAR antibodies were from Cell Signaling Technology; anti-DAP1, phospho-PKD1 (S738/742) and phospho-PKD1 (S916) antibodies were from Abcam; anti-ATF4 antibody was from Santa Cruz Biotechnology; and anti-GAPDH, anti-PKD2 and anti-PKD3 antibodies were from GeneTex. Anti-DnaK antibody was obtained from ENZO. Anti-SubAB antibody was prepared as previously described (Yahiro et al., 2006 (link)). PKC inhibitor Gö6976 was obtained from LC Laboratories; PKC activator PMA, PKC inhibitor Gö6983, PKD/PKCμ inhibitor CID755673, PKA activator 8Br-cAMP, Thapsigargin (TG), CaM kinase II inhibitor KN-93 were from Sigma Aldrich; and PKA inhibitor 14–22 Amide was from Calbiochem; PKC inhibitor Bisindolylmaleimide II was from ALEXIS Biochemicals; and ROCK II inhibitor Y-27632 was from Cayman Chemical.
Pkc inhibitor g 6976
Manufactured by LC Laboratories
Gö6976 is a potent and selective inhibitor of the Protein Kinase C (PKC) enzyme. It acts by competitively inhibiting the ATP-binding site of PKC. Gö6976 demonstrates high specificity towards the classical PKC isoforms, including PKC-alpha, -beta I, and -gamma.
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Lab products found in correlation
Anti bip grp78, by BD (2 mentions)
Anti cleaved poly adp ribose polymerase cparp, by Cell Signaling Technology (2 mentions)
Pkc inhibitor g 6983, by Merck Group (2 mentions)
Anti phospho eif2α, by Cell Signaling Technology (2 mentions)
Anti eif2α, by Cell Signaling Technology (2 mentions)
Monoclonal anti α tubulin antibody, by Merck Group (1 mentions)
Anti g3bp1, by BD (1 mentions)
Thapsigargin tg, by Merck Group (1 mentions)
Anti pkd1, by Cell Signaling Technology (1 mentions)
Anti tiar antibodies, by Cell Signaling Technology (1 mentions)
Anti atf4 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti dnak antibody, by Enzo Life Sciences (1 mentions)
Anti pkcδ, by BD (1 mentions)
Anti p erk, by Cell Signaling Technology (1 mentions)
Anti bax, by BD (1 mentions)
Ru486, by Cayman Chemical (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody, by GeneTex (1 mentions)
Anti perk, by BD (1 mentions)
Anti bak, by BD (1 mentions)
2 protocols using pkc inhibitor g 6976
1
Antibody Reagents for Protein Analysis
2
Investigating Apoptosis Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-Bak, anti-BiP/Grp78, anti-Bax, and anti-ERK monoclonal antibodies were from BD Bioscience; anti-PERK, anti-Bcl-xL, anti-Bim, Anti-Bak, anti-Bax, anti-eIF2α, anti-phospho-eIF2α, anti-phospho-ERK, anti-cleaved caspase 6 (cCas6) and anti-cleaved poly(ADP-ribose) polymerase (cPARP) antibodies were from Cell Signaling Technology; and anti-GAPDH antibodies were from GeneTex. Anti-SubAB antibodies were prepared as previously described6 (link). PKC inhibitor Gö6976 and ERK inhibitor U0126 were obtained from LC Laboratories. SB23590 was from WAKO. PKC activators (PMA and prostratin), Thapsigargin, and PKC inhibitor Gö6983 were from Sigma Aldrich. Bryostatin 1 was from Santa Cruz Biotechnology. PKC inhibitor Bisindolylmaleimide II (Bis II) was from ALEXIS Biochemicals. PKC activator Ingenol-3-angelate (I3AG), SP600125, and steroid antagonist RU486 were from Cayman Chemical. Dexamethasone sodium phosphate (Dx) was from FujiPhama. Prednisolone (P) was from SHIONOGI & CO., Ltd. Methylprednisolone Succinate Na (MP) was from Sawai Pharmaceutical Co., Ltd. Hydrocortisone (HC) was from Taisho Pharmaceutical Co., Ltd.
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