Dioleoyl phosphatidylcholine, dioleoyl phosphatidylserine, egg SM, dipalmitoyl phosphatidylserine, GM1, NBD‐PC, and Bodipy‐Chol were purchased from Avanti Polar Lipids. TR–DHPE, AF–CTxB, and OG–DHPE were purchased from Invitrogen. Other chemicals, including NaCl, CaCl2, MgCl2, 4‐(2‐Hydroxyethyl)piperazine‐1‐ethanesulfonic acid (HEPES), hexadecane, silicone oil AR 20, methyl‐β‐cyclodextrin, and SMase from Bacillus cereus were obtained from Sigma‐Aldrich. All the lipids were dissolved in chloroform or the mixture of chloroform and methanol, and stored in a refrigerator at −20 °C. NaCl, CaCl2, MgCl2, and HEPES were dissolved in distilled water at concentrations of 100 × 10−3, 5 × 10−3, 2 × 10−3, and 10 × 10−3m , respectively, to prepare an aqueous buffer solution, followed by titration to pH 7.4 with a 0.5 m NaOH solution. The aqueous buffer solution was stored in the refrigerator at 4 °C. AF–CTxB and SMase were dissolved in the aqueous buffer at concentrations of 50 µg mL−1 and 5 U mL−1, respectively, and they were stored in the refrigerator at 4 °C.
Dipalmitoyl phosphatidylserine
Manufactured by Avanti Polar Lipids
Dipalmitoyl phosphatidylserine is a synthetic phospholipid compound. It is a key component of cell membranes and plays a role in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
4 2 hydroxyethyl piperazine 1 ethanesulfonic acid hepes, by Merck Group (1 mentions)
Silicone oil ar20, by Merck Group (1 mentions)
Tr dhpe, by Thermo Fisher Scientific (1 mentions)
Smase from bacillus cereus, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Methyl β cyclodextrin, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Cacl2, by Merck Group (1 mentions)
Hexadecane, by Merck Group (1 mentions)
Egg sm, by Avanti Polar Lipids (1 mentions)
Dioleoylphosphatidylcholine, by Avanti Polar Lipids (1 mentions)
Dioleoyl phosphatidylserine, by Avanti Polar Lipids (1 mentions)
Nbd pc, by Avanti Polar Lipids (1 mentions)
Bodipy chol, by Avanti Polar Lipids (1 mentions)
Aztreonam, by Merck Group (1 mentions)
Carbonyl cyanide m chlorophenyl hydrazine, by Merck Group (1 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
E coli polar lipid extract, by Avanti Polar Lipids (1 mentions)
Dipalmitoylphosphatidylethanolamine, by Avanti Polar Lipids (1 mentions)
2 protocols using dipalmitoyl phosphatidylserine
1
Lipid Reagent Preparation and Characterization
2
Fluorescence Labeling of Bacterial Proteins
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aTC was from Spectrum Chemical Manufacturing Corp. [32P]PO4 was from PerkinElmer, MP Biomedicals, or American Radiolabeled Chemicals Inc. TNBS and DFDNB were purchased from Thermo Fisher Scientific and Sigma-Aldrich, respectively. MPB and AMS were purchased from Invitrogen Molecular Probes Biotechnology; n-OG and βME were purchased from Sigma-Aldrich. Carbonyl cyanide m-chlorophenyl hydrazine came from Sigma-Aldrich. E. coli polar lipid extract, E. coli CL, dipalmitoylphosphatidylethanolamine (DPPE), and dipalmitoylphosphatidylserine (DPPS) were purchased from Avanti Polar Lipids (Alabaster, AL). Streptavidin-conjugated Alexa Fluor 430, avidin–horseradish peroxidase, and SuperSignal West Pico chemiluminescent substrate came from Thermo Fisher Scientific. Aztreonam was obtained from Sigma-Aldrich. Biotinylated Ro09-0198 was a gift from M. Umeda (Kyoto University, Japan). Antibodies directed against SecA and PldA were gifts from K.-i. Nishiyama (Iwate University, Morioka, Japan) and J. Tommassen (Utrecht University, The Netherlands), respectively. NR12S and pyrene PA probes were gifts from A. Klymchenko (University of Strasbourg, France), O. Kucherak (Charles University, Prague), and Y. Niko (Kochi University, Japan).
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