Blood samples from the HELPcB program were analyzed with the Sysmex XN-9000 hematology analyzer (Sysmex Deutschland GmbH) and immunophenotypic analysis was performed as previously described (29 ) . In brief, EDTA anticoagulated whole blood was incubated for 20 min at room temperature with fluorescently labeled antibody pairs (CD3/CD4, CD3/CD8, CD3/CD19, CD3/CD16ϩCD56) and isotype-matched controls (IgG1 FITC/IgG2a PE, all from Becton Dickinson). Erythrocytes were lysed with BD FACS lysing solution and leukocytes were analyzed on a FACSCalibur with use of the BD Simulset software (Becton Dickinson). LDCs of validation set II and IV were determined either (a) with an automated hematology analyzer (Coulter AcT diff2, Beckman Coulter), (b) by microscopic analysis of blood smears, and/or (c) by immunophenotyping and flow cytometric analysis on a Navios flow cytometer (Beckman Coulter). Blood samples of validation set III were analyzed with an Abbott Cell-Dyn Emerald hematology system (Abbott Laboratories).
Xn 9000 hematology analyzer
Manufactured by Sysmex
Sourced in Japan, Germany
The XN-9000 is a fully automated hematology analyzer designed for high-volume clinical laboratories. It performs comprehensive blood cell analysis, including red blood cells, white blood cells, and platelets. The XN-9000 utilizes advanced technologies to provide accurate and reliable results, supporting the diagnosis and monitoring of various hematological conditions.
Automatically generated - may contain errors
Lab products found in correlation
Navios flow cytometer, by Beckman Coulter (3 mentions)
Facs lysing solution, by BD (1 mentions)
Coulter ac t diff 2, by Beckman Coulter (1 mentions)
Cd3 cd19, by BD (1 mentions)
Cs 5100, by Sysmex (1 mentions)
Crp 2 latex x2, by Denka Seiken (1 mentions)
Cobas c702 automatic analyzer, by Roche (1 mentions)
Advia centaur xpt, by Siemens (1 mentions)
Advia chemistry xpt, by Siemens (1 mentions)
Atellica analyzer, by Siemens (1 mentions)
Advia 2120i, by Siemens (1 mentions)
Xn 9000, by Sysmex (1 mentions)
Immulite 2000 xpi, by Siemens (1 mentions)
Cobas c501, by Roche (1 mentions)
Vitek 2 system, by bioMérieux (1 mentions)
Bactec plus aerobic f, by BD (1 mentions)
Maldi tof ms, by bioMérieux (1 mentions)
Cobas 8000, by Roche (1 mentions)
Icp ms, by Agilent Technologies (1 mentions)
Cobas 8000 c702, by Roche (1 mentions)
31 protocols using xn 9000 hematology analyzer
1
Hematology and Immunophenotypic Analysis
2
Blood Sample Analysis Protocol for Clinical Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For all patients, blood samples were collected on the same day and were assessed in the Clinical Laboratory of The First Hospital of Jilin University. Antibodies were tested by immunofluorescence or immunoblotting assays (AMA, anti-sp100, and anti-gp210) using a EUROLineMaster Plus-A fully automatic immunoblotting instrument. Immunoglobulin M was measured by turbidimetric inhibition immunoassay. The blood biochemistry indices were detected by an automatic biochemical analyzer (7600-210, Hitachi, Japan). Specifically, serum ALB level was measured by bromopotassium phenol green method (normal values: 40.0-55.0 g/L); serum TBIL level was measured by enzymatic method involving bilirubin oxidase (normal values: 6.8-30.0 μmol/L). Complete blood count was measured using a Sysmex XN-9000 hematology analyzer (Sysmex Corporation, Kobe, Japan). The coagulation tests were performed by the clotting method on the automatic coagulometer "Sysmex CS-5100" (Sysmex Corporation, Kobe, Japan). All assays were measured according to the manufacturer's instructions.
3
Comprehensive Blood and Semen Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood samples were taken from the median cubital vein of all involved participants and placed in tubes containing ethylenediaminetetraacetic acid. All blood samples were analyzed within 2 h with SYSMEX XN-9000 hematology analyzer from Japan. The routine blood test of platelet included mean platelet volume (MPV) and platelet count (PLT). For semen analysis, the semen samples were obtained by masturbation, requiring sexual abstinence for 3-4 days. The semen samples were analyzed within 1 h after liquefaction at room temperature with SQA-V automated sperm quality analyzer from Israel according to WHO guidelines (2010). The primary parameters of semen analysis included semen volume, sperm progressive motility, sperm concentration, and sperm morphology.
4
Hematological Profiling of NPC Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected in tubes containing ethylenediaminetetraacetic acid. Hematology test included measurement of the levels of hemoglobin (Hb), hematocrit, plateletcrit, RDW, neutrophil, lymphocyte, and platelets using the Sysmex XN-9000 hematology analyzer (Sysmex Corp, Kobe, Japan) for 30 minutes after the blood draw was completed. All the patients with NPC were clinically staged in accordance with the 7th edition of the American Joint Committee on Cancer tumor, node, metastasis (TNM) staging manual.
5
Preoperative Inflammatory Marker Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Preoperative levels of inflammatory markers were determined with peripheral blood samples collected within 5 days before surgery. CRP was measured by latex-enhanced turbidimetric immunoassay, using a CRP latex (II) X2 (Denka Seiken Inc., Tokyo, Japan) with high-sensitivity application. Assay range of CRP was from 0.005 to 16 mg/dL. ESR was measured by TEST-1 SDL automated erythroid sediment rate analyzer (Alifax Inc., Polverara, Italy). WBC was determined using Sysmex XN-9000 hematology analyzer (Sysmex Inc., Hyogo, Japan). The NLR was calculated on the basis of WBC differential count with dividing the neutrophil count by the lymphocyte count.
6
Biomarker Analysis for Pulmonary Embolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood specimens were harvested immediately after the diagnosis of PE was confirmed, or after a routine antenatal visit. Specimens of blood were centrifuged at 4000 revolutions per minute for 10 minutes and analyzed within 2 hours. Briefly, a Sysmex XN9000 hematology analyzer (Sysmex Corporation, Japan) was used for blood routine analysis. Fib, APTT, PT, and D-D levels were analyzed using a Werfen ACLTOP700 hematology analyzer (Werfen Corporation, Spain). In addition, we collected 5 mL of venous blood in a gold tube with a clot activator and gel, and the serum was collected to measure ALB, Cr, and Ca levels using a Cobas C702 automatic analyzer (Roche Corporation, Switzerland). FAR was determined by dividing the plasma Fib concentration by the serum ALB levels. In our clinical laboratory, all tests were performed in accordance with ISO15189.
7
Linearity Assessment of HbA1c Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Linearity was assessed according to the CLSI EP06-A2 guidelines. One sample with a high value (20.23% HbA1c) and one sample with a low value (2.96% HbA1c) were mixed at different proportions (0%, 6.25%, 12.5%, 18.75%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 100%) to obtain 11 concentration gradient samples. Each sample was measured on the BS-600M for three times consecutively to obtain measured values and compare them with expected values. The expected HbA1c values were calculated according the following formula: Expected value = (%HbA1c × VL × HbL + %HbA1c × VH × HbH)/(VL × HbL + VH × HbH). VL/H: volume of the sample with low/high HbA1c value; HbL/H: Hb concentration of the sample with low/high HbA1c value. Hb concentration was measured by a Sysmex XN9000 hematology analyzer (Sysmex Co., Japan).
8
Inflammatory Markers in Hospital Admissions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All data were extracted from medical records. Age, sex, place of residence before admission, and the presence of delirium during the hospital stay were documented. Severity of comorbidities on admission was calculated using the Charlson Comorbidity Index (CCI).18 (link) The total WBC count, neutrophil count, lymphocyte count, and CRP level within 24 h after admission were recorded. The NLR was calculated by dividing the neutrophil count by the lymphocyte count. WBC, neutrophil, and lymphocyte counts were determined on a Sysmex XN-9000 hematology analyzer with flow cytometry (Sysmex Corporation, Kobe, Hyogo Prefecture, Japan). CRP was determined with the CRPL3 assay (immunoturbidimetric method) on a Cobas 8000 c701/702 analyzer (Roche Diagnostics, Rotkreuz, Switzerland).
9
Anti-PF4 Antibody Screening Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All participants with detectable anti-PF4 antibodies received CBC testing at the time that they were scheduled for their third anti-PF4 antibody testing. Blood samples were collected in an EDTA tube. CBC was assessed using the automated Sysmex® XN-9000 hematology analyzer (Sysmex, Kobe, Japan).
10
Analytical Methods for Clinical Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the Jeroen Bosch Hospital, CRP was measured on the Advia Chemistry XPT (Siemens Healthineers, Erlangen, Germany), ferritin and vitamin B12 were measured on the Advia Centaur XPT (Siemens Healthineers), folic acid was measured on the Immulite 2000 XPi (Siemens Healthineers), and hematological parameters were measured on the Advia 2120i (Siemens Healthineers). At the St Jansdal Hospital, CRP and ferritin were measured on the Atellica Analyzer (Siemens Healthineers), and hematological parameters were measured on the Sysmex XN-9000 (Sysmex Corporation, Hyogo, Japan). At Medlon BV, CRP and ferritin were measured with the C702 and e801 module (COBAS 8000 routine chemistry analyzer, Roche Diagnostics, Mannheim, Germany) respectively, and hematological parameters were measured on the XN-9000 hematology analyzer (Sysmex Corporation).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!