First, the leaves were cleaned and rinsed with water. Afterward, the leaves of P. scutellaria were dried for preparation of the plant extract in the oven within the temperature of 50 o C. The leaves of P. scutellaria were collected from Lenteng Agung Area, South Jakarta, Indonesia. For the extract preparation, 50 g of dry powder of the leaves were placed in a 1000 mL of Erlenmeyer containing 500 mL of ethanol solvent. The content in the Erlenmeyer was mixed at first with stirrer stick and being macerated for 24 hours at room temperature. The extract was then filtered using Whatman® paper No.2 (Cat. Number: 28419866, Sigma-Aldrich, USA) Then, the solvent was evaporated by using a rotary evaporator and vacuum (Buchi R-205 and V-500) at temperature 60 o C. The obtained extract was weighed for 16 mg and dissolved in 10 mL 1% DMSO.
V 500
Manufactured by Büchi
Sourced in United States, Switzerland
The V-500 is a vacuum pump designed for a wide range of laboratory applications. It provides reliable and efficient vacuum generation for various processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using v 500
1
Ethanolic Extraction of P. scutellaria Leaves
2
Preparation of Liposomal Dispersions
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A liposomal dispersion was prepared following the standard thin-film hydration method [26] .
In brief, approximatively 2 g soy phosphatidylcholine (S-100) were dissolved into 50 mL of methanol in a round bottom flask. The organic solvent was removed by controlled vacuum evaporation (25°C; 1 hour; 60-65 mBar final vacuum) employing a Büchi rotary evaporator system (model R-124), equipped with a water bath (model B-480) and vacuum pump (model V-500; Büchi Labortechnik AG, Flawil, Switzerland). Large liposomes dispersion was obtained by reconstituting the lipid film obtained after solvent removal with 50 mL PBS. The liposomal dispersion was subsequently extruded throw 800 nm (4 cycles) and 400 nm (4 cycles) polycarbonate filters (Whatman International Ltd., Bucking-hamshire, UK) in order to obtain a homogeneous dispersion of medium-sized liposomes (average diameter approximately 400 nm). Prior to analysis, one mL of the liposomal dispersion was mixed with 1 mL of API solution inside an Eppendorf vial (samples caf5, hc5, ibu6 and ket6 in Table 2).
Samples were incubated for 10 min prior to analysis.
In brief, approximatively 2 g soy phosphatidylcholine (S-100) were dissolved into 50 mL of methanol in a round bottom flask. The organic solvent was removed by controlled vacuum evaporation (25°C; 1 hour; 60-65 mBar final vacuum) employing a Büchi rotary evaporator system (model R-124), equipped with a water bath (model B-480) and vacuum pump (model V-500; Büchi Labortechnik AG, Flawil, Switzerland). Large liposomes dispersion was obtained by reconstituting the lipid film obtained after solvent removal with 50 mL PBS. The liposomal dispersion was subsequently extruded throw 800 nm (4 cycles) and 400 nm (4 cycles) polycarbonate filters (Whatman International Ltd., Bucking-hamshire, UK) in order to obtain a homogeneous dispersion of medium-sized liposomes (average diameter approximately 400 nm). Prior to analysis, one mL of the liposomal dispersion was mixed with 1 mL of API solution inside an Eppendorf vial (samples caf5, hc5, ibu6 and ket6 in Table 2).
Samples were incubated for 10 min prior to analysis.
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