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Bt474 human breast cancer cells

Manufactured by American Type Culture Collection
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The BT474 human breast cancer cells are a well-established cell line derived from a human breast carcinoma. These cells are intended for use in research applications to study breast cancer biology and development.

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Lab products found in correlation

Skbr3 human breast cancer cells, by American Type Culture Collection (1 mentions) Dmem f12, by Merck Group (1 mentions) Nucleocounter nc 200, by ChemoMetec (1 mentions) Hybri care medium, by American Type Culture Collection (1 mentions) Mda mb 468 cell line, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Welgene (1 mentions)

Close spelling variants of this product

BT474 cells BT474

4 protocols using bt474 human breast cancer cells

1

Establishing Trastuzumab-Resistant Breast Cancer Cells

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BT474 human breast cancer cells (HER2-overexpression) were obtained from the American Type Culture Collection (catalog number HTB-20, ATCC) and were cultured in RPMI 1640 supplemented with 10% FBS. Wild-type (WT) SKBR3 human breast cancer cells (catalog number HTB-30, ATCC) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS). Trastuzumab-resistant (TR) SKBR3 cells were established by continuous culture of WT SKBR3 cells in the presence of 5 μg/ml trastuzumab for 6 months in a humidified atmosphere of 5% CO2 and 95% air at 37 °C according to our previous reports1 (link),3 (link).
Bai W., Peng H., Zhang J., Zhao Y., Li Z., Feng X., Zhang J., Liang F., Wang L., Zhang N., Li Y., Zhu H, & Ji Q. (2022). LINC00589-dominated ceRNA networks regulate multiple chemoresistance and cancer stem cell-like properties in HER2+ breast cancer. NPJ Breast Cancer, 8, 115.
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2

Culturing and Inoculating BT-474 Breast Cancer Cells

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BT-474 human breast cancer cells were purchased from the American Type Culture Collection, authenticated and tested to be negative for common mouse pathogens and for mycoplasma. The cells were cultured in DMEM/F-12 (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (iFBS, Sigma-Aldrich) in a humidified incubator at +37°C and 5% CO2. 1 × 106 of BT-474 cells suspended in 20 μl of 1x phosphate-buffered saline (PBS) was used per inoculation to the mice. Before and after the inoculation, cell viability was determined (NucleoCounter®, NC-200, ChemoMetec) and it was above 80%.
Kähkönen T.E., Suominen M.I., Mäki-Jouppila J.H., Halleen J.M., Tanaka A., Seiler M, & Bernoulli J. (2019). Human Immune System Increases Breast Cancer-Induced Osteoblastic Bone Growth in a Humanized Mouse Model without Affecting Normal Bone. Journal of Immunology Research, 2019, 4260987.
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3

Cultivation of Human Breast Cancer Cells

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HER2-positive BT474 human breast cancer cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) and subcultured in Hybri-Care medium (ATCC) with 10% FBS at 37°C in a humidified incubator, and passaged every 4–5 days. The HER2-negative MDA-MB-468 cell line was obtained from ATCC and used as a control.4 (link)
Ding H., Gangalum P.R., Galstyan A., Fox I., Patil R., Hubbard P., Murali R., Ljubimova J.Y, & Holler E. (2016). HER2-positive breast cancer targeting and treatment by a peptide-conjugated mini nanodrug. Nanomedicine : nanotechnology, biology, and medicine, 13(2), 631-639.
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4

Culturing BT474 Breast Cancer Cells

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BT474 human breast cancer cells (ATCC, American Type Culture Collection; Manassas, VA, USA) were cultured in RPMI-1640 medium containing 50 U/ml penicillin, 50 mg/ml streptomycin and 10% fetal bovine serum (FBS; Welgene, Daegu, Korea) at 37°C in an atmosphere of 5% CO2.
SEO H.S., KU J.M., CHOI H.S., CHOI Y.K., WOO J.K., KIM M., KIM I., NA C.H., HUR H., JANG B.H., SHIN Y.C, & KO S.G. (2016). Quercetin induces caspase-dependent extrinsic apoptosis through inhibition of signal transducer and activator of transcription 3 signaling in HER2-overexpressing BT-474 breast cancer cells. Oncology Reports, 36(1), 31-42.
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