M phosphoric acid

Isoproterenol hydrochloride and protease inhibitor cocktail were purchased from Sigma Chemical Co., St. Louis, MO, USA; while the chemicals used for biochemical assays were products of E. Merck Ltd., Mumbai, India. All other chemicals and reagents used were procured from Hi-Media, Mumbai, India. Thio-barbituric acid (TBA), sodium dodecyl sulphate (SDS), Ellman’s reagent and m-phosphoric acid were obtained from E. Merck Ltd., Mumbai, India. While assay kits for CK-MB, LDH, AST and ALT were purchased from Teco Diagnostic,USA; for total cholesterol, Triglyceride and HDL-C, kits were obtained from Span diagnostics Pvt. Ltd., Surat, India. The TNF-α and IL-6 specific ELISA kits were from Ray Biotech, Inc., USA and cardiac troponin-I (cTnI) kit was procured from the Ortho-Clinical Diagnostics, Inc. New York, USA. While Bisacrylamide, Tris hydroxymethyl aminomethane (Tris), glycine, N,N, NU,NU-tetramethylethyldiamide, ammonium persulfate, glycerol, ultra pure urea, 2D cleanup kit and 2D Quant kit were purchased from GE Healthcare, Amersham, Freiburg, Germany; Acrylamide, dithiothreitol, 3–3–1-propane-sulfonate, agarose and Iodoacetamide were purchased from Fluka BioChemika, Buchs, Switzerland.