For immuno uorescence staining, hiPSCs colonies and RPCs were xed and permeabilized using 4% paraformaldehyde and 1% Triton X-100. Samples were blocked with 5% bovine serum albumin (BSA) for 1h at room temperature. hiPSCs colonies were stained overnight with unconjugated anti-OCT4A Rabbit mAb (Cell Signaling Technology, 1:200), anti-Nanog (Cell Signaling Technology, 1:200), anti-PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), anti-Nephrin (Thermo Scienti c, 10 μg/mL), and anti-Sinaptopodin (Thermo Scienti c, 20 μg/mL). The RPCs were stained overnight with primary antibodies to PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), Nephrin (Thermo Scienti c, 10 μg/mL), and Sinaptopodin (Thermo Scienti c, 20 μg/mL). On the next day, samples were stained with the Alexa Fluor 488 secondary antibody (Life Technologies, Carlsbad, CA, USA, 1:1000). Subsequently, slides were mounted with Vecta Shield Antifade Mounting Medium (Vector Laboratories, Burlingame, CA, USA) containing DAPI, and images were acquired using the EVOS FL Imaging System (Thermo Fisher Scienti c, Waltham, MA, USA).
Sinaptopodin
Manufactured by Thermo Fisher Scientific
Sinaptopodin is a structural protein found in the podocytes of the kidney. It plays a role in the maintenance and regulation of the actin cytoskeleton within these cells. Sinaptopodin is essential for the proper function and integrity of the glomerular filtration barrier.
Automatically generated - may contain errors
Lab products found in correlation
Anti oct4a rabbit mab, by Cell Signaling Technology (2 mentions)
Anti nanog, by Cell Signaling Technology (2 mentions)
Anti pax2, by Abcam (2 mentions)
Alexa fluor 488 secondary antibody, by Thermo Fisher Scientific (2 mentions)
Vectashield antifade mounting medium, by Vector Laboratories (2 mentions)
Anti nephrin, by Thermo Fisher Scientific (2 mentions)
Anti sinaptopodin, by Thermo Fisher Scientific (2 mentions)
Nephrin, by Thermo Fisher Scientific (2 mentions)
2 protocols using sinaptopodin
1
Immunofluorescence Staining of hiPSCs and RPCs
2
Immunofluorescence Staining of hiPSCs and RPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For immuno uorescence staining, hiPSCs colonies and RPCs were xed and permeabilized using 4% paraformaldehyde and 1% Triton X-100. Samples were blocked with 5% bovine serum albumin (BSA) for 1h at room temperature. hiPSCs colonies were stained overnight with unconjugated anti-OCT4A Rabbit mAb (Cell Signaling Technology, 1:200), anti-Nanog (Cell Signaling Technology, 1:200), anti-PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), anti-Nephrin (Thermo Scienti c, 10 μg/mL), and anti-Sinaptopodin (Thermo Scienti c, 20 μg/mL). The RPCs were stained overnight with primary antibodies to PAX2 (Abcam, 1:100), WT-1 (Abcam, 1:100), Nephrin (Thermo Scienti c, 10 μg/mL), and Sinaptopodin (Thermo Scienti c, 20 μg/mL). On the next day, samples were stained with the Alexa Fluor 488 secondary antibody (Life Technologies, Carlsbad, CA, USA, 1:1000). Subsequently, slides were mounted with Vecta Shield Antifade Mounting Medium (Vector Laboratories, Burlingame, CA, USA) containing DAPI, and images were acquired using the EVOS FL Imaging System (Thermo Fisher Scienti c, Waltham, MA, USA).
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