Nanopet ct

Manufactured by Bioscan

Sourced in United States

The NanoPET/CT is a preclinical imaging system that combines positron emission tomography (PET) and computed tomography (CT) technologies. It is designed for small animal research, providing high-resolution functional and anatomical imaging capabilities.

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Lab products found in correlation

Pmod version 3, by PMOD Technologies (1 mentions) Vivoquant software, by Invicro (1 mentions) Nucline 1, by Bioscan (1 mentions) Pmod software, by PMOD Technologies (1 mentions)

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NanoPET/CT small-animal imaging system (3 citations)

7 protocols using nanopet ct

FDG PET/CT Imaging of Tumor Xenografts

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In total, 15 mice (n = 5 for each group) were used for FDG PET/CT imaging after the therapy completion. Mice were fasted at least 6 h before intravenous administration of ¹⁸F-FDG. Mice were injected with 18.5 MBq of ¹⁸F-FDG via tail vein and PET/CT images were acquired after 60 min after injection (Nano PET/CT, Bioscan). During the image acquisition, the mice were anaesthetised (5%) and sustained (1%~2%) with isoflurane in a 7:3 mixture of N₂/O₂.
Analysis of the PET/CT images was performed using PMOD version 3.9 (PMOD Technologies, https://www.pmod.com). Polygonal regions of interest were manually drawn with care to include whole tumour volumes. The radiotracer uptake in each ROI was estimated as the standardised uptake value (SUV), which was calculated as decay-corrected activity per millilitre of tissue divided by injected radiotracer activity per body mass ([kBq/mL]/[kBq/g]). Metabolic tumour volume (MTV) was measured with fixed absolute threshold and SUV 0.02 was used as threshold.

Yoon J.K., Byeon H.E., Ko S.A., Park B.N., An Y.S., Lee H.Y., Lee Y.W, & Lee S.J. (2020). Cell cycle synchronisation using thiazolidinediones affects cellular glucose metabolism and enhances the therapeutic effect of 2-deoxyglucose in colon cancer. Scientific Reports, 10, 4713.

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Small Animal PET/CT Imaging of 18F-FDG

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In addition to the SPECT/CT, a PET/CT scan with ¹⁸F-FDG was done at the same day. All animals were fasted for 24 hours before imaging to follow the protocol of current clinical practice36 (link), thus minimize ¹⁸F-FDG uptake in normal myocardium. Rats were anesthetized with 1.5–2% isoflurane in 100% oxygen (2 L/min flow rate). Following a 20-min CT scan for attenuation correction, the PET data acquisition was started at the time of intravenous injection with ¹⁸F-FDG (1.20 ± 0.09 mCi). The 3D static images were collected for 90 minutes with an energy window of 400–600 keV.
All PET scans were performed using a small animal PET scanner, NanoPET/CT (Bioscan Inc, USA), which provides a minimum axial coverage of 9.48 cm, a 12.3 mm transaxial field of view, 0.3 mm sampling distance, 0.58 mm in-plane reconstructed resolution and 7.7% of absolute sensitivity at the center of field of view for an energy window at 250–750 keV.

Lee M.S., Park H.S., Lee B.C., Jung J.H., Yoo J.S, & Kim S.E. (2016). Identification of Angiogenesis Rich-Viable Myocardium using RGD Dimer based SPECT after Myocardial Infarction. Scientific Reports, 6, 27520.

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Isoflurane-Anesthetized Rodent CT Imaging

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Animals were anesthetized with 2% isoflurane/balance O2. Imaging was performed using the CT component of a NanoPET/CT (Bioscan, Washington, DC) scanner and image analysis was performed using VivoQuant software (inviCRO, Boston, MA) using a Hounsfield Unit windowing technique, normalized for all scans. Volumetric segmentation was performed using a neighborhood threshold.

Xie H., Hanai J.I., Ren J.G., Kats L., Burgess K., Bhargava P., Signoretti S., Billiard J., Duffy K.J., Grant A., Wang X., Lorkiewicz P.K., Schatzman S., Bousamra M I.I., Lane A.N., Higashi R.M., Fan T.W., Pandolfi P.P., Sukhatme V.P, & Seth P. (2014). Targeting lactate dehydrogenase-A inhibits tumorigenesis and tumor progression in mouse models of lung cancer and impacts tumor initiating cells. Cell metabolism, 19(5), 795-809.

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Quantitative PET/CT Imaging in Preclinical Oncology

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PET/CT scans were performed on the Bioscan NanoPET/CT at the Longwood Small Animal Imaging Facility. This PET scanner is equipped with a dedicated isoflurane anesthesia system, temperature controlled platform, cardiac gating, and respiratory gating. PET scanning was performed on anesthetized animals lying motionless on a table, after Retro-orbital IV injection of 0.1 to 10 mCi of F18-FDG PET radioisotope, while being imaged with a coincidence camera. The mice were imaged after a pre-determined “washout” period (30-60 min). Individual mice were first scanned pre-treatment and then 40 hours after the treatment regimen was initiated (see dosing schedule methods). For quantitative analysis, the standardized uptake value (SUV) normalized to body weight in the tumor was calculated using SUV= ACvoi (kBq/ml)/(FDGdose(MBq)/BW(kg)) where ACvoi is the average activity concentration in the tumor volume (or the maximum value); FDGdose is the dose of F18-FDG administered; and BW is the body weight. For evaluating tumors the highest SUV in the tumor was taken as the SUVmax.

Malone C.F., Fromm J.A., Maertens O., DeRaedt T., Ingraham R, & Cichowski K. (2014). Defining key signaling nodes and therapeutic biomarkers in NF1-mutant cancers. Cancer discovery, 4(9), 1062-1073.

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Anesthetic-Assisted Small Animal PET/CT

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Before imaging, the rats were anesthetized with 5% isoflurane and sustained with 5% isoflurane in a 6:4 mixture of N2/O2. PET/CT imaging was performed in a dedicated small animal PET/CT (NanoPET/CT, Bioscan Inc., Washington DC, USA) with a 10 cm axial field of view (FOV) and a 12 cm transaxial

Bang J.I., Jung I.S., Song Y.S., Park H.S., Moon B.S., Lee B.C, & Kim S.E. (2016). PET imaging of dopamine transporters with [(18)F]FE-PE2I: Effects of anti-Parkinsonian drugs. Nuclear medicine and biology, 43(2).

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Small Animal PET/CT Dual Modality Imaging

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Dual modality PET/CT acquisitions were performed using a small animal dedicated system (nanoPET/CT, Bioscan) and proprietary software (Nucline 1.07, Bioscan). A thirty (30) min-long PET acquisition was performed from 2.5 to 3 h p.i. with 1-3 coincidence mode and image reconstruction was performed using constructor's OSEM algorithm. A CT acquisition was then performed using 180 projections (600 ms each) at 45 kVp and reconstructed using dedicated software (Nucline 1.07, Bioscan). PET images were quantified using InVivoScope 2.0 software (Bioscan) and 2 volumes of interest (VOIs) were drawn. A first spherical volume of interest (radius: 0.5 mm) was centered on aortic arch lesions as identified by CT signal enhancement [17] (link) and a second VOI matched the prevertebral course of the thoracic aorta above the diaphragm muscle. Activity concentration in the VOI was expressed as mean standardized uptake value (SUV_mean), i.e. tissue activity concentration (MBq·mL⁻¹)/injected dose (MBq) · body weight (g).

Toczek J., Broisat A., Perret P., Desruet M.D., Fagret D., Riou L.M, & Ghezzi C. (2014). Periaortic Brown Adipose Tissue as a Major Determinant of [18F]-Fluorodeoxyglucose Vascular Uptake in Atherosclerosis-Prone, ApoE−/− Mice. PLoS ONE, 9(7), e99441.

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Tracking Brain Neuroreceptor Dynamics

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Health SD rat was intravenously injected with 37 MBq/0.1 mL of [⁶⁸Ga]IPCAT-NOTA 24 through lateral tail veins and anesthetized with isoflurane gas (3% isoflurane in 50% oxygen, 1 mL/min). The imaging of [⁶⁸Ga]IPCAT-NOTA 24 was acquired for 60 minutes using nanoPET/CT (BioScan, Inc., Washington, DC, USA) with dynamic image and semi-quantiﬁcation was processed by PMOD software (PMOD Technologies Ltd., Zürich, Switzerland). We set up a PMOD template for brain including striatum and cerebellum, respectively. Cerebellum served as the reference region, and region of interest (ROI) was calculated from the average radioactivity with the following equation: (target - reference)/reference.

Farn S.S., Chang K.W., Lin W.C., Yu H.M., Lin K.L., Tseng Y.C., Chang Y., Yu C.S, & Lin W.J. (2021). Synthesis, Radiolabeling, and Preliminary in vivo Evaluation of [68Ga] IPCAT-NOTA as an Imaging Agent for Dopamine Transporter. Drug Design, Development and Therapy, 15, 2577-2591.

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