After the 15 days of rest designed to allow the development of PTSD, corticosterone (CORT), corticotropin-releasing hormone (CRH), and adrenocorticotroic hormone (ACTH) in the plasma, and 5-HT concentration in the brain tissue were assayed according to a previously described procedure [31 (link)]. Four rats from each group were deeply anesthetized through inhalation with isoflurane (1.2%), and were sacrificed one day after behavioral testing. The plasma was quickly collected via the abdominal aorta, and the medial prefrontal cortex (mPFC), hippocampus (HPC), striatum (STR), and amygdala (AMYG) were rapidly removed from the rat brain in a randomized order. Special care was taken to avoid pre-sacrifice stress and the rats were killed rapidly. The CORT, CRH, ACTH, and 5HT concentration were evaluated by a competitive enzyme-linked immunoassays (ELISAs) using a CORT antibody (Novus Biologicals, LLC., Littleton, CO, USA), a CRH antibody (Novus Biologicals), a ACTH antibody (Novus Biologicals), and a 5-HT antibody (Abcam, Cambridge, MA, USA) according to the manufacturer's protocols.
5 ht antibody
Manufactured by Abcam
Sourced in United States
The 5-HT antibody is a laboratory reagent used to detect and measure the presence of serotonin, also known as 5-hydroxytryptamine (5-HT), in biological samples. This antibody can be used in various immunoassay techniques to quantify 5-HT levels in cells, tissues, or body fluids.
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Lab products found in correlation
2 protocols using 5 ht antibody
1
Neurochemical Changes in PTSD Model
2
PTSD Biomarkers in Rat Brain
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After the 15 days of rest designed to allow the development of PTSD, corticosterone (CORT) and tryptophan (TRP) in the plasma and 5-HT concentration in the brain tissue were assayed according to a previously described procedure [10 (link), 25 (link)]. Four rats from experimental group were deeply anesthetized through inhalation with isoflurane (1.2 %) and were sacrificed one day after behavioral testing. The plasma was rapidly collected via the abdominal aorta, and the medial prefrontal cortex, amygdala, striatum, and hippocampus were quickly removed from the rat brain in a randomized order. Special care was taken to avoid presacrifice stress and the rats were killed quickly. CORT, TRP, and 5-HT levels were evaluated by a competitive enzyme-linked immunoassays (ELISAs) using a TRP antibody (Biocompare, San Francisco, CA, USA), a 5-HT antibody (Abcam, Cambridge, MA, USA), and a CORT antibody (Novus Biologicals, LLC, Littleton, CO, USA) according to the manufacturer's protocols.
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