Leupeptin (L2884), AgNO3, sodium borohydride, Nα‐benzoyl‐dl ‐arginine‐4‐nitroanilide hydrochloride (BAPA), trypsin from porcine pancreas type IX‐S, titanium(IV) oxide, zinc oxide, 1,1ʹ‐CDI, triethylamine, APTES, DMSO, acetonitrile (ACN), formic acid, acetic acid, O‐(1H‐6‐chlorobenzotriazole‐1‐yl)‐1,1,3,3‐tetramethyluronium‐hexafluorophosphate, N,N‐diisopropylethylamine, dichloromethane, piperidine, acetic anhydride, trifluoroacetic acid (TFA), triisopropylsilane, Fmoc–Leu–OH, Fmoc–Phe–OH and Boc–Ahx–OH were all purchased from Sigma‐Aldrich. WorkBeads™ 40/1000 ACT was a kind gift from Bio‐Works Sweden AB, Uppsala.
Fmoc leu oh
Manufactured by Merck Group
Sourced in Germany
Fmoc-Leu-OH is a chemical compound used as a reagent in solid-phase peptide synthesis. It is a protected amino acid derivative with the Fmoc (fluorenylmethyloxycarbonyl) protecting group attached to the amino group of leucine.
Automatically generated - may contain errors
Lab products found in correlation
Piperidine, by Merck Group (3 mentions)
Fmoc phe oh, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Trifluoroacetic acid, by Merck Group (2 mentions)
Triisopropylsilane, by Merck Group (2 mentions)
N n dimethylformamide (dmf), by Merck Group (2 mentions)
Fmoc lys boc oh, by Merck Group (2 mentions)
Trypsin, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Agno3, by Merck Group (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
N n diisopropylethylamine, by Merck Group (1 mentions)
Acetic anhydride, by Merck Group (1 mentions)
Aptes, by Merck Group (1 mentions)
Zinc oxide, by Merck Group (1 mentions)
Titanium 4 oxide, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
5 protocols using fmoc leu oh
1
Peptide Synthesis and Characterization
2
Peptide Synthesis and Purification
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Chemicals were ordered from commercial sources as follows: Fmoc-Phe-Wang, Fmoc-Phe-OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Lys(Boc)-OH, Fmoc-Ala-OH, Fmoc-Gly-OH, 4-aminophenylacetic acid, DMF, DCM, TIPS, piperidine (Sigma Aldrich, Germany); chloroplatinic acid (8 wt% in water), HOBT, TBTU, diethyl ether (Fluka, Buchs, Switzerland); 2-propanol, DMSO (VWR, Leuven, Netherlands); TFA (Merck, KGaA, Darmstadt, Germany); DIEA (Roth, Karlsruhe, Germany); water (Millipore grade) (laboratory purification system).
3
Synthetic Lipid Membrane Components
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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanoloamine
(POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
(POPG), 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol
(DPPG), and 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-glycerol (CL) were purchased from Avanti Polar
Lipids Inc. Phosphate buffer saline, all amino acid derivatives (Fmoc-d Phe-OH, Fmoc-Leu-OH, Fmoc-Dab(Boc)-OH), coupling reagent 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethylaminium
tetrafluoroborate (TBTU), and N,N-diisopropylethylamine (DIPEA) were purchased from Sigma-Aldrich.
All solvents were obtained from Avantor Performance Materials Poland
S.A. The water was purified through the Milli-Q system (resistivity
18.2 MΩ × cm). In all experiments, we have used an aqueous
solution of 0.01 M phosphate buffer saline (PBS) adjusted to pH =
7.4 unless otherwise stated.
(POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol
(POPG), 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol
(DPPG), and 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-glycerol (CL) were purchased from Avanti Polar
Lipids Inc. Phosphate buffer saline, all amino acid derivatives (Fmoc-
tetrafluoroborate (TBTU), and N,N-diisopropylethylamine (DIPEA) were purchased from Sigma-Aldrich.
All solvents were obtained from Avantor Performance Materials Poland
S.A. The water was purified through the Milli-Q system (resistivity
18.2 MΩ × cm). In all experiments, we have used an aqueous
solution of 0.01 M phosphate buffer saline (PBS) adjusted to pH =
7.4 unless otherwise stated.
4
Dendrimer-based Peptide Conjugation
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Polyamidoamine dendrimer ethylenediamine core generation 2 solution (G2 PAMAM), N,N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), Fmoc-Leu-OH, Fmoc-Phe-OH, Fmoc-Lys (Boc)-OH, piperidine, triisopropylsilane (TIS), trifluoroaceticacid (TFA), diisopropylethylamine (DIPEA), dimethylsulfoxide (DMSO), deuterium oxide (D2O), Ampicillin, Kanamycin, n-phenyl-1-naphthylamine (NPN), and crystal violet were purchased from Sigma-Aldrich Korea (Seoul, Republic of Korea). 2-(1h-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) and hydroxybenzotriazole (HOBt) were purchased from Anaspec (San Jose, CA, USA). Paraformaldehyde (PFA) was purchased from Thermo Scientific and silicon wafers from TWOWIZ Wafermart. NIH3T3 cells were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea).
5
Stable Isotope Labeled Peptide Synthesis
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Fmoc-protected
amino acids, DIC and HBTU, and
peptide grade dimethylformamide were purchased from AGTC Bioproducts
(Hessle, U.K.). H-Rink Amide-Chemmatrix resin was acquired from PCAS
BioMatrix Inc. (Saint-Jean-sur-Richelieu, QC). 15N-labeled
amino acids Fmoc-Asn(Trt)-OH and Fmoc-Leu-OH were obtained from Sigma-Aldrich.
H-Leu-OH, which was labeled with 13C at the α-carbon,
was acquired from Campro Scientific (Berlin, Germany) and subsequently
Fmoc-protected following standard procedures. All other chemicals
were purchased form Fisher Scientific (Loughborough, U.K.). Water
was purified with a Synergy ultraviolet (UV) water purification system
from Millipore. Peptide concentrations were determined by UV absorbance
[λ280 (Trp) = 5690 mol–1 cm–1; λ280 (Tyr) = 1280 mol–1 cm–1] using a NanoDrop 2000 spectrophotometer
from Thermo Scientific.
amino acids, DIC and HBTU, and
peptide grade dimethylformamide were purchased from AGTC Bioproducts
(Hessle, U.K.). H-Rink Amide-Chemmatrix resin was acquired from PCAS
BioMatrix Inc. (Saint-Jean-sur-Richelieu, QC). 15N-labeled
amino acids Fmoc-Asn(Trt)-OH and Fmoc-Leu-OH were obtained from Sigma-Aldrich.
H-Leu-OH, which was labeled with 13C at the α-carbon,
was acquired from Campro Scientific (Berlin, Germany) and subsequently
Fmoc-protected following standard procedures. All other chemicals
were purchased form Fisher Scientific (Loughborough, U.K.). Water
was purified with a Synergy ultraviolet (UV) water purification system
from Millipore. Peptide concentrations were determined by UV absorbance
[λ280 (Trp) = 5690 mol–1 cm–1; λ280 (Tyr) = 1280 mol–1 cm–1] using a NanoDrop 2000 spectrophotometer
from Thermo Scientific.
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