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Ab188877

Manufactured by Abcam
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Ab188877 is a polyclonal antibody produced in rabbit targeting the EGFR protein. It is designed for use in various immunoassay applications.

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Lab products found in correlation

Fv3000, by Olympus (1 mentions) Ab24525, by Abcam (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Bovine serum albumin (bsa), by Abcam (1 mentions) Sudan black, by Merck Group (1 mentions) Ab11575, by Abcam (1 mentions) Ab75872, by Abcam (1 mentions) Ab66043, by Abcam (1 mentions)

3 protocols using ab188877

1

Cardiac Cell Immunostaining and Confocal Imaging

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At 48 h post-fixation, hearts were sectioned into 200-µm slices. For staining, slices were first incubated for 10 min in blocking solution (3% normal donkey serum (NDS) in PBST), followed by primary antibody staining overnight at 4 °C using the following antibodies: anti-vimentin (ab24525), anti-cardiac troponin I (ab188877) or anti-PGP9.5 (ab108986), purchased from Abcam at 1:200 dilution in blocking solution. Slices were then washed twice in PBST, then stained with secondary antibodies (1 mg ml−1) at 1:500 dilution for 3 h at room temperature using the following: F(ab’)2 anti-chicken 488 (703-546-155) and anti-rabbit 647 (711-606-152) purchased from Jackson ImmunoResearch Laboratories. The slices were then stained with DAPI and washed three times with PBST (30 min per wash). Sections were mounted onto slides and mounted with exPROTOS. Slices were imaged on a confocal microscope (Olympus FV3000).
Hsueh B., Chen R., Jo Y., Tang D., Raffiee M., Kim Y.S., Inoue M., Randles S., Ramakrishnan C., Patel S., Kim D.K., Liu T.X., Kim S.H., Tan L., Mortazavi L., Cordero A., Shi J., Zhao M., Ho T.T., Crow A., Yoo A.C., Raja C., Evans K., Bernstein D., Zeineh M., Goubran M, & Deisseroth K. (2023). Cardiogenic control of affective behavioural state. Nature, 615(7951), 292-299.
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2

Cardiac Tissue Immunostaining Protocol

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Briefly, cardiac sections were deparrafinized and rehydrated, and antigens were retrieved using Retreivit 6 (InnoGennex). Sections were treated with 2% Sudan Black (Sigma-Aldrich) in 70% ethanol to quench autofluorescence and blocked with 3% BSA (Sigma-Aldrich). Slides were incubated with primary antibodies against TGF-β 1/2/3 (sc-7892, Santa Cruz Biotechnology), TGF-β (ab66043, Abcam), Troponin I (ab188877, Abcam), Integrin β3 (ab75872, Abcam), and Laminin (ab11575, Abcam) in PBS with 1% BSA. AlexaFluor-labeled secondary antibodies with ‒488 or ‒555 conjugates were used (Thermo Fisher Scientific). Images were obtained from 5 fields of 4 separate hearts for each staining condition.
Flevaris P., Khan S.S., Eren M., Schuldt A.J., Shah S.J., Lee D.C., Gupta S., Shapiro A.D., Burridge P.W., Ghosh A.K, & Vaughan D.E. (2017). Plasminogen Activator Inhibitor Type I Controls Cardiomyocyte Transforming Growth Factor-β and Cardiac Fibrosis. Circulation, 136(7), 664-679.
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3

Serum cTnI Assay for Myocardial I/R Injury

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Serum cTnI level was detected by an ELISA kit (ab188877, Abcam) following the manufacturer's instructions to evaluate myocardial I/R injury.
Guan L., Yu Z., Che Z., Zhang H., Yu Y., Yang D., Qian D., Chen R, & Yu M. (2022). Experimental diabetes exacerbates autophagic flux impairment during myocardial I/R injury through calpain‐mediated cleavage of Atg5/LAMP2. Journal of Cellular and Molecular Medicine, 27(2), 232-245.
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