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Anti cyclin a antibody

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The Anti-CYCLIN A antibody is a laboratory reagent used for detection and analysis of the CYCLIN A protein. CYCLIN A is a key regulator of the cell cycle and plays a crucial role in the progression of cells through the G1/S and G2/M transitions. This antibody can be used in various immunoassay techniques to identify and quantify CYCLIN A levels in biological samples.

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Lab products found in correlation

Alexa fluor 594 goat anti rabbit, by Thermo Fisher Scientific (2 mentions) Anti dna rna hybrid s9.6 antibody, by Kerafast (2 mentions) Anti parp1 antibody, by Abcam (2 mentions) Anti xrcc1 antibody, by Cell Signaling Technology (2 mentions) Anti rpa70 rpa1 antibody, by Cell Signaling Technology (2 mentions) Anti dna polymerase beta antibody, by Abcam (2 mentions) Rnase h enzyme, by New England Biolabs (2 mentions) Anti nucleolin antibody, by Abcam (2 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (2 mentions) S9.6 antibody, by Kerafast (2 mentions) Lsm 700, by Zeiss (1 mentions)

2 protocols using anti cyclin a antibody

1

Immunofluorescence Analysis of DNA-RNA Hybrids

Cited 1 time
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Immunofluorescence using S9.6 antibody (Kerafast INC.) was performed as previously described (21 (link)). Cells were incubated with primary antibodies, anti-DNA-RNA hybrid [S9.6] antibody (1:500, Kerafast INC.), anti-nucleolin antibody (1:1000, Abcam Cat # ab22758), anti-PARP1 antibody (1:1000, Abcam Cat # ab32138 and 1:500 Cat # ab191217), anti- XRCC1 antibody (1:1000, Cell Signaling Technology Cat #27350), anti-DNA Polymerase beta antibody (1:1000, Abcam Cat # ab26343), anti-CYCLIN A antibody (1:200, Sigma Cat #C4710), and anti-RPA70/RPA1 antibody (1:50, Cell Signaling Technology Cat #2267S) followed by incubation in secondary antibodies Alexa Fluor 488 goat anti-mouse (1:1000, Invitrogen Cat #A11034) or Alexa Fluor 594 goat anti-rabbit (1:1000, Invitrogen Cat #A11005). DNA was stained with DAPI. Images were captured at 40X magnification with a Zeiss Confocal Microscope (Zeiss LSM 700). Fiji software (version: 2.0.0-rc-69/1.52i, open source image processing software) used for analysis of images. As a control, cells were treated with 10 units of RNase H enzyme (New England Biolabs Cat # M0297L), prior to staining with S9.6 antibody.
Safari M., Litman T., Robey R.W., Aguilera A., Chakraborty A.R., Reinhold W.C., Basseville A., Petrukhin L., Scotto L., O'Connor O.A., Pommier Y., Fojo A.T, & Bates S.E. (2021). R-Loop–Mediated ssDNA Breaks Accumulate Following Short-Term Exposure to the HDAC Inhibitor Romidepsin. Molecular Cancer Research, 19(8), 1361-1374.
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2

Immunofluorescence Quantification of DNA-RNA Hybrids

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence using S9.6 antibody (Kerafast Inc.) was performed as previously described (21 (link)). Cells were incubated with primary antibodies, anti-DNA–RNA hybrid (S9.6) antibody (1:500, Kerafast Cat # ENH001), anti-nucleolin antibody (1:1,000, Abcam Cat # ab22758), anti-PARP1 antibody (1:1,000, Abcam Cat # ab32138 and 1:500 Cat # ab191217), anti-XRCC1 antibody (1:1,000, Cell Signaling Technology Cat #27350), anti-DNA Polymerase beta antibody (1:1,000, Abcam Cat # ab26343), anti-CYCLIN A antibody (1:200, Sigma Cat #C4710), and anti-RPA70/RPA1 antibody (1:50, Cell Signaling Technology Cat #2267S) followed by incubation in secondary antibodies Alexa Fluor 488 goat anti-mouse (1:1,000, Invitrogen Cat #A11034) or Alexa Fluor 594 goat anti-rabbit (1:1,000, Invitrogen Cat #A11005). DNA was stained with DAPI. Images were captured at ×40 magnification with a Zeiss Confocal Microscope (Zeiss LSM 700). Fiji software (version: 2.0.0-rc-69/1.52i, open source image processing software) used for analysis of images. As a control, cells were treated with 10 U of RNase H enzyme (New England Biolabs Cat # M0297L), before staining with S9.6 antibody.
Safari M., Litman T., Robey R.W., Aguilera A., Chakraborty A.R., Reinhold W.C., Basseville A., Petrukhin L., Scotto L., O'Connor O.A., Pommier Y., Fojo A.T, & Bates S.E. (2021). R-Loop–Mediated ssDNA Breaks Accumulate Following Short-Term Exposure to the HDAC Inhibitor Romidepsin. Molecular Cancer Research, 19(8), 1361-1374.
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